摘要
从茯砖茶中分离出冠突散囊菌,对其进行液体发酵培养,制备成粗酶液。在不同条件下处理粗酶液,运用3,5-二硝基水杨酸(DNS)还原糖法测定纤维素酶活性。试验结果表明,冠突散囊菌所产纤维素酶酶活最佳温度是50℃,最佳pH值是4.8;在30～50℃,pH4～6范围内有较高的稳定性;Ca2+、Mg2+离子对酶活有激活作用,Mn2+、Zn2+、Pb2+离子对酶活有抑制作用。
Eurotium cristatum isolated from brick tea were enlarged to foster by liquid fermentation and then crude enzyme was prepared from the culture of Eurotium cristatum. Cellulase activity of the crude enzyme liquid was measured by 3,5-dinitrosalicylic acid method under different conditions. The results showed that the optimum temperature for enzymatic reaction was 50℃, and the optimum reactive pH value was at 4.8. The cellulase activity was stabile under conditions of 30-50℃ and pH 4.0-6.0. The cellulase activity was stimulated by Ca^2＋, Mg^2＋ ions and inhibited by Mn^2＋, Zn^2＋. Pb^2＋ ions. The research will be significance for production of brick tea.
出处
《茶叶科学》
CAS
CSCD
北大核心
2010年第1期57-62,共6页
Journal of Tea Science
基金
湖南省重大科技专项(2008FJ1005)
关键词
茯砖茶
冠突散囊菌
纤维素酶
酶学性质
brick tea, Eurotiumcristatum, cellulose, enzymatic property
