摘要
[目的]提高草地早熟禾的转化频率。[方法]以草地早熟禾的丛生芽芽尖为转化受体,研究了影响农杆菌转化草地早熟禾频率的有关因素。[结果]农杆菌悬浮液浓度OD600=0.6,并添加0.1 mmol/L乙酰丁香酮,在50 kPa真空处理5 m in,获得的抗性芽比例较高,最大达15.19%。[结论]农杆菌悬浮液浓度、真空渗透处理及乙酰丁香酮的诱导均能影响草地早熟禾的转化频率。
[ Objective ] The study aimed to enhance the transformation frequency of Kentucky bluegrass. [ Method J With shoot tips of multiple bud multiple as transformation target, the factors which influenced transformation of Kentucky bluegrass mediated by Agrobacterium tumefaciens were studied. [ Result] Higher percentage of resistant buds （ hygromycin-resistant buds / infected buds） was obtained under the following conditions ： shoot tips were infected in Agrobacterium suspension of OD600 =0. 6 supplemented with 0.1 mmol/L acetosyrigone for 5 minites under 50 kPa pressure, and the highest percentage of resistant buds reached 15.19%. [ Conclusion ] The transformation efficiency was affected by the concentration of Agrobacterium suspension, vacuum infiltration and aeetosyringone （AS） induction.
出处
《安徽农业科学》
CAS
北大核心
2010年第1期58-59,62,共3页
Journal of Anhui Agricultural Sciences
关键词
草地早熟禾
农杆菌
转化
Kentucky bluegrass
Agrobacterium tumefaeiens
Transformation
