摘要
目的比较两种人大肠癌细胞株的转移潜能及其相关性．方法利用裸鼠实验性肝转移模型比较人大肠癌ＨＴ２９与ＬｏＶｏ细胞株的转移潜能；定量检测两种细胞株层粘连蛋白受体（ＬＮＲ）、上皮性钙调蛋白（Ｅｃａｄ）及Ⅳ型胶原酶的表达；用原位分子杂交及免疫组化检测两细胞株ＣＤ４４Ｖ６在蛋白质水平及ｍＲＮＡ水平的表达．结果ＬｏＶｏ细胞株为人大肠癌高转移株，裸鼠实验性肝转移率为１００％；ＨＴ２９细胞株转移相对较低，裸鼠肝内转移率为４２９％．定量检测发现，ＬｏＶｏ细胞几乎不表达Ｅｃａｄ，ＨＴ２９细胞膜上弱表达Ｅｃａｄ．ＬｏＶｏ表达ＬＮＲ与Ⅳ型胶原酶高于ＨＴ２９．ＬｏＶｏ细胞内ＣＤ４４Ｖ６的表达在蛋白质水平与ｍＲＮＡ水平均高于ＨＴ２９细胞．结论ＬｏＶｏ细胞株为人大肠癌高转移株，ＨＴ２９细胞株转移力相对较低；ＬＮＲ、Ｅｃａｄ、Ⅳ型胶原酶及ＣＤ４４Ｖ６可作为大肠癌细胞转移潜能的相关指标．
IM To compare the metastatic potentials between two human colorectal carcinoma cell lines and to study their features related to metastasis. METHODS Experimental metastatic potentials of two human colorectal carcinoma (HCC) cell lines, HT29 and LoVo, were compared by using nude mice model of HCC liver metastasis. The expressions of Ecadherin, LNR and type Ⅳ collagenase (Ⅳ col) of these HCC cell lines in vitro were observed quantitatively. The cells expressing CD44V6 in mRNA and protein were detected by in vitro hybridization and immunohistochemistry. RESULTS The nude mice had 100% liver metastasis which demonstrated that LoVo is a high metastatic HCC cell line. However, HT29 is a relatively low metastatic HCC cell line since only 429% of nude mice transplanted HT29 cells had liver metastasis. The expressions of Ecad, LNR and Ⅳ col of HT29 and LoVo cells in vitro were detected quantitatively which showed that HT29 cells had weak Ecadherin expression on cell membrane while LoVo cells showed almost no expression. The expressions of LNR and Ⅳ col in LoVo cells both were higher than that in HT29 cells. The expressions of CD44V6 of LoVo cells were higher than that of HT29 cells in either mRNA or protein. CONCLUSION LoVo is a high metastatic HCC cell line, while HT29 is a relatively low metastatic HCC cell line. Ecadherin, LNR, Ⅳ col and CD44V6 can be used to determine the metastatic potential of HCC cells. .
