摘要
目的 探讨 6 70 0 0层黏连蛋白受体 (LN R)在喉癌侵袭转移过程中的作用。方法 采用逆转录聚合酶链反应 (RT PCR)方法检测 2 0例喉鳞状细胞癌及其正常组织标本中 6 70 0 0LN RmRNA的表达 ,分析其水平与临床病理特征间的关系。采用流式细胞术检测人喉癌细胞系AMC HN 8细胞膜表面 6 70 0 0LN R的表达。应用体外黏附实验和Boyden小室体外侵袭实验 ,观察 6 70 0 0LN R单克隆抗体 (MLuC5 )对AMC HN 8细胞黏附和侵袭能力的影响。结果 人喉鳞状细胞癌肿瘤组织中6 70 0 0LN RmRNA的表达显著高于正常组织 (P <0 .0 5 ) ;伴颈淋巴结转移的喉鳞癌组织与无颈淋巴结转移的喉鳞癌组织相比 ,伴颈淋巴结转移的喉鳞癌组织 6 70 0 0LN RmRNA的表达水平更高 (P <0 .0 5 ) ;6 70 0 0LN RmRNA的表达与肿瘤分化程度密切相关 ,分化差者表达水平更高 (P <0 .0 5 )。流式细胞术显示 ,AMC HN 8细胞 6 70 0 0LN R阳性表达率为 (80 .9± 0 .9) %。MLuC5抑制了AMC HN 8细胞对层黏连蛋白 (LN)的黏附 ,MLuC5处理 6 0min和 12 0min时 ,黏附抑制率分别为 5 7.1%和 6 3.2 %。MLuC5降低了AMC HN 8细胞对基膜的侵袭能力。结论 6 70 0 0LN R高表达的喉鳞癌细胞侵袭性更强 ,6 70 0 0LN
Objective To investigate the role of 67 000 laminin receptor (LN-R) in the processes of invasion and metastasis of laryngeal squamous cell carcinoma. Methods Expression of 67 000 LN-R mRNA in 20 cases with laryngeal squamous cell carcinomas and corresponding normal tissues was determined by RT-PCR, and the relationship between its expression level and patients′ clinicopathological features was analyzed. Expression of 67 000 LN-R on the surface of AMC-HN-8 laryngeal carcinoma cells was examined by flow cytometry. The effect of 67 000 LN-R monoclonal antibody (MLuC5) on the adhesive and invasive abilities was observed by adhesion test and Boyden chamber invasiveness test in vitro. Results The expression level of 67 000 LN-R mRNA in human laryngeal squamous cell carcinoma was significantly higher than that in normal tissues(P<0.05). The expression level of 67 000 LN-R mRNA in laryngeal squamous cell carcinomas with cervical lymph node metastases was higher than in those without cervical lymph node metastases(P<0.05). There was a significant negative correlation between the expression level of 67 000 LN-R mRNA and the degree of tumor differentiation, the level being higher in poorly differentiated tumors(P<0.05). Flow cytometry showed that (80.9±0.9)% of AMC-HN-8 cells expressed 67 000 LN-R. MLuC5 inhibited the adhesion of AMC-HN-8 cells on LN, and after treated with MLuC5 for 60 and 120 minutes, the adhesion inhibition rate was 57.1% and 63.2%, respectively. The invasive ability to artificial basement membrane was reduced by MLuC5. Conclusion Laryngeal carcinoma overexpressing 67 000 LN-R has stronger invasiveness, and 67 000 LN-R monoclonal antibody may contribute to prevent metastasis of laryngeal carcinoma.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2004年第9期539-542,共4页
Chinese Journal of Oncology
基金
上海市科技发展基金资助项目 ( 0 0 41190 17)
关键词
LN-R
HN
喉癌
表达
ML
层黏连蛋白受体
喉鳞癌
RNA
细胞
黏附
Laryngeal neoplasms
Laminin recptor
Neoplasm metastasis
Reverse transcription polymerase chain reaction
