摘要
以籼稻单倍体绿芽无性系为材料，研究秋水仙碱等处理对水稻植株染色体二倍化的影响．结果显示：（1）以500mg／L秋水仙碱溶液振荡处理籼稻单倍体绿芽48h，其二倍化率达60％～75％，处理时间过长或处理浓度过高．虽植株染色体二倍率有所提高，但二倍体植株的得率下降；（2）秋水仙碱在愈伤组织阶段处理，其染色体二倍率远高于非愈伤组织化的绿芽二倍率，高达96％～100％，其实未经处理的绿芽愈伤组织再生植株也能获得较高的加倍率；（3）在培养基中添加多效唑（2．5mg／L）也能增加籼稻植株染色体二倍率，由原来的3．3％提高到6．6％．
Haploid green shoots from long term subculture were used to study the effects of colchicine on diploidization of haploid green shoot clones. The results indicated that: (1 )When the haploid green shoots were shooked with sterile colchicine solution at concentration of 500 mg/L for 48 hours, the frequency of diploidization was 60% ~ 75%. The longer the treatment time or the greater the concentration of colchicine solution was used, the gnater the frequency of diploidization was reduced; (2)The frequency of diploidization at callus stagecould be as high as 96% ~ 100%. However, the frequency of diploidization of green shoot callus could be 74%; (3 )The frequency of diploidization in Indica rice increased from 3.3% to 6.6% with MET(2.5 mg/L)solution.
出处
《应用与环境生物学报》
CAS
CSCD
1998年第3期232-234,共3页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金!39570443
浙江省自然科学基金!395051
