摘要
将分化出的冬小麦花粉植株的苗龄分为绿芽,1cm绿苗、3cm绿苗,经3—8℃的冷库内生长66天,这些不同苗龄的绿苗均生长3cm左右,越夏后生根培养一个月,其生根率分别为96.4％、72.9％、70.6％,以绿芽越夏后生根率最高。移栽成活后处于分蘖盛期的单倍体花粉植株,用0.04％的秋水仙碱与1.5％的二甲基亚砜混合液在18℃条件下浸泡分蘖节6—8h,加倍率可达82.9-87.7％。
Age of winter wheat plantlets regenerated from anthers was called green bud, 1cm height plantlets and 3cm height plantlets. Growing for 66 days at 3—8℃, these green plantlets of different age increased about 3 cm at height. After they passed summer, they were cultured 1 month for root production. The root productive freguency was 96.4％, 72.9％ and 70.6％ respectivly, among which the highest was the plantlets at green bud age. At peak tillering stage of survival plants, chromosome double frequency reached from 82.9％ to 87.7％ by steeping tiller nodles for 6—8hr. in a mixed solution of 0.04％ calchicine and 1.5% dimethyl sulfaxide.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
1992年第1期4-7,共4页
Journal of Hebei Agricultural University
关键词
苗龄
小麦
染色体加倍
夏季
summering
plantlet age
chromosome double
