摘要
山梨醇和甘露醇作碳源，对籼稻品系ＴＣ９０及其花培单倍体幼穗愈伤组织诱导、植株分化与染色体自然加倍的研究结果表明，２％和４％的山梨醇作唯一碳源时，可维持外植体生存２５ｄ以上，绿茵再生频率分别为５２．６％和９０．０％，单倍体自然加倍率达３０．５％。２％或４％的甘露醇作碳源时，外植体均不能生长，逐渐变得苍白、干瘪，培养７ｄ后失去分化再生能力。
The effects of sorbitol and mannitol as carbon source on callus induction, plantlet regeneration and haploidchromosome doubling in diploid and haploid young panicle culture of indica rice line TC90 were studied. The results showedthat 2% or 4% sorbitol as medium carbon source could efficiently maintain explants alive at least for 25 days, and plantlet regeneration frequency reached 52. 6% and 90. 0% respectively, and haploid doubling rate reached as high as 30. 5%. On thecontrary, the explants did not produce any callus, became pale, shrunk and died out after being cultured seven days when 2%or 4% mannitol used as carbon source.
出处
《中国水稻科学》
CAS
CSCD
北大核心
1999年第4期248-250,共3页
Chinese Journal of Rice Science
关键词
山梨醇
自然加倍
体细胞培养
水稻
sorbitol
auto-doubling
somatic cell culture
rice
