摘要
应用同位素标志法,研究了赤潮异弯藻、亚历山大藻和中肋骨条藻核酸和蛋白质合成动态对芘的响应变化。结果表明:(1)芘处理对3种藻的96 h半抑制剂量分别为0.071、0.107和0.097 mg/L,3种海洋赤潮微藻对芘的敏感性依次是赤潮异弯藻、中肋骨条藻和亚历山大藻。(2)低浓度的芘处理对3种藻的生长与DNA的合成表现出刺激作用,高浓度则表现出抑制作用。(3)芘处理抑制3种藻的RNA和蛋白质的合成。随着芘浓度的增大,3种海洋赤潮微藻RNA和蛋白质的合成速度下降,其中赤潮异弯藻合成速度的下降幅度明显大中肋骨条藻和亚历山大藻,表明赤潮异弯藻RNA和蛋白质的合成对芘的敏感性高于中肋骨条藻和亚历山大藻。
The responses of Heterosigma akashiwo, Alexandrium tamarense and Skeletonema costatum to pyrene stress in protein and nucleic acid synthesis were studied with the isotope-tracing methods. The results showed that the order of sensitivity from high to low in three red-tide microalgae to pyrene was H. akashiwo, S. costatum and A. tamarense. Higher concentration pyrone inhibited the growth and DNA synthesis in throe species of rod-tide microalgae, however, lower concentration pyrene could stimulated their growth and DNA synthesis. Meanwhile, the RNA and protein synthesis in three species of marine red-tide microalgae is decreased with the increased pyrene concentration, but the decrease in H. akashiwo was more obvious than A. tamarense and S. costatum. Therefore, the sensitivity of RNA and protein synthesis to pyrene in H. akashiwo was higher than A. tamarense and S. costatum.
出处
《海洋环境科学》
CAS
CSCD
北大核心
2008年第4期320-322,347,共4页
Marine Environmental Science
基金
国家自然科学基金(30270258)
山东省优秀中青年科学家科研奖励基金(03BS120)
关键词
海洋赤潮微藻
蛋白质
核酸
合成作用
芘
marine red-tide microalgae
nucleic acid
protein
synthesis
pyrone
