摘要
研究原料乳中蜡样芽孢杆菌的潜在威胁,对我国10个省市原料乳中蜡样芽孢杆菌的污染状况进行调查,并利用PCR扩增技术及血平板检测的方法分析乳源性蜡样芽孢杆菌中的毒性分布。结果表明,原料乳中蜡样芽孢杆菌检出率为33%,检出水平为1×103～1×106CFU/ml,检出的蜡样芽孢杆菌中25株(75.8%)能产生溶血素BL,24株(72.7%)含有bceT基因,至少产生一种毒素的菌株有31株占检出菌总数的93.9%。研究结果证实我国原料乳中的蜡样芽孢杆菌污染状况不容乐观,严格监控原料乳中的蜡样芽孢杆菌污染,对生产出优质乳制品具有重要意义。
In order to evaluate the toxigenic potential of Bacillus cereus in row milk,100 samples of row milk from 10 cities of China were screened,and Bacillus cereus were found in 33（33%） samples with the concentration within 1 × 103～1 × 106 CFU/ ml.Using PCR the isolates were checked for the presence of the hblA gene of the hemolysin BL（HBL） complex and the bceT genes of the enterotoxin T.HBL was detected by blood plate.Totally 25（75.8%） of the 33 B.cereus isolates produced the hemolysin BL,while 24（72.7%） isolates possessed the bceT gene,and as a result,31（93.9%） B.cereus isolates produced at least one toxin.The results suggest that it is necessary to monitor the Bacillus cereus contamination in row milk.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第22期236-239,共4页
Food Science
基金
"十一五"国家科技支撑计划项目(2006BAD04A08)
