摘要
目的研究三七总皂苷(PNS)对急性心肌梗死(AMI)后左室重构(LVRM)大鼠血清肿瘤坏死因子-α(TNF-α)与基质金属蛋白酶-2(MMP-2)表达的影响并探讨其作用机制。方法除假手术组外采用结扎大鼠左冠状动脉前降支的方法建立AMI模型,术后24h后随机分为对照组和实验组,连续4周分别灌胃给予生理盐水、福辛普利和PNS低、中、高剂量,观察PNS对病鼠左室舒张末期内径(宽度)(LVIDd)、左室收缩末期内径(宽度)(LVIDs)、左室射血分数(EF)、左室收缩百分率(FS)、二尖瓣口舒张早期血流速度(MV)、心率等反映心功能变化指标及TNF-α与MMP-2表达的影响。结果PNS中、高剂量组的EF、FS及MV指标均显著提高(P<0.01或0.05);PNS低、中、高剂量组均能明显抑制TNF-α与MMP-2的表达(P<0.05)。结论PNS能够抑制TNF-α与MMP-2的分泌与释放,通过提高左室收缩和舒张功能,降低外周阻力,减轻AMI后LVRM大鼠的心肌缺血再灌注损伤,对病鼠AMI后LVRM心功能有保护和改善作用。
Objective To observe the effects of panax notoginsenoside （PNS） on tumor necrosis factor- α（TNF-α） and matrix metalloproteinases-2 （MMP-2） expressions in rats with post-myocardial infarction ventricular remodeling and explore the mechanism. Methods Rat models of acute infarction ventricular （AMI） were established by ligation of the left anterior descending coronary artery. Twenty-four hours after the operation, the rats were randomized into control and experimental groups for intragastric administration of normal saline （control）, fosinopril and PNS at the low, medium and high doses for 4 consecutive weeks. The effects of PNS on the cardiac function index including the left ventricular end-diastolic dimension （LVIDd）, left ventricular end-systolic diameter （LVIDs）, ejection fraction （EF）, percentage of left ventricular systole （FS）, mitral early diastolic flow velocity mouth （MV）, and heart rate （HR） were observed, and the changes in TNF-α and MMP-2 expression were detected after post-myocardial infarction ventricular remodeling. Results Compared with the control group, PNS at the medium and high doses produced significant improvements in the EF, FS and MV of the rats （P〈0.01 or 0.05）. TNF-α and MMP-2 expressions were significantly decreased by PNS treatment at low, medium and high doses （P〈0.01）. Conclusion PNS can inhibit or reduce the expression of TNF-α and MMP-2, thereby enhancing left ventricular systolic and diastolic functions, decreasing peripheral resistance, and improving the cardiac function of rats with post-myocardial infarction left ventricular remodeling.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第10期2048-2050,共3页
Journal of Southern Medical University
基金
广东省科技计划项目(83100)
广东省中医药局科研基金(1060060
2008005)
广东省医院药学研究基金(2009A008)
广州市中医药中西医结合科研基金(2008A15)
