摘要
目的研究三七总皂苷对氧化型低密度脂蛋白(ox-LDL)损伤血管内皮细胞的保护作用,以探讨其治疗动脉硬化闭塞症(ASO)的机制。方法以培养原代人脐静脉内皮细胞(HUVEC)作为靶细胞,用ox-LDL造成HUVEC损伤模型,以三七总皂苷进行干预。光镜下观察细胞形态变化,MTT法检测细胞活性;采用蛋白定量法检测HUVEC与单核细胞的黏附率;用流式细胞仪测定HUVEC的细胞间黏附分子-1(ICAM-1)的蛋白表达。结果ox-LDL作用HUVEC后12、24 h时,HUVEC损伤明显,细胞活性显著降低,人单核细胞与HUVEC的黏附率显著升高,HUVEC的ICAM-1的蛋白表达水平也明显升高,均明显高于正常对照组(P<0.05,P<0.01),而三七总皂苷能使ox-LDL损伤的HUVEC形态趋于正常,活性增强,并明显降低人单核细胞与HUVEC的黏附率,显著降低ICAM-1蛋白的表达水平,与ox-LDL组比较差异有统计学意义(P<0.05,P<0.01),这种作用随着剂量的增加而增强。结论三七总皂苷能通过下调内皮细胞表面黏附分子的表达抑制单核-血管内皮细胞黏附,从而发挥对血管内皮细胞的保护作用,这可能是其治疗ASO的机制之一。
Objective To study the protective effect of panax notoginseng saponins （PNS） on human umbilical vascular endothelial cells （HUVECs） injury induced by oxidized low-density lipoprotein （ox-LDL） for investigating the mechanism of PNS in treating arteriosclerosis obliterans （ASO）. Methods Taking the cultured HU- VECs as target cells, ox-LDL was used to establish a model of injured HUVEC and it was then intervened by PNS. The morphologic changes of HUVEC were observed under light microscope ; activity of cells was determined by MTT method; the adhesive percentage between ox-LDL treated HUVEC and monocyte determined by protein quantification and the protein expression of intercellular adhesion molecule-1 （ICAM-1） determined by flow cytometry. Resuits At the time points of HUVEC being treated with ox-LDL （ 100 mg/L） for 12 h and 24 h, significant injury of HUVEC was shown, its activity reduced, the adhesion rate with monocytes elevated, and the protein expression of ICAM-1 in HUVEC increased significantly （P 〈 0.05, P 〈 0.01 ）. PNS showed significant effect in reversing all the above changes, as compared with the control group （without PNS treaded）, respective significant difference was shown in all the four indexes （P 〈 0.05, P 〈 0.01 ）. Conclusion PNS has a protective effect on endothelial cells injury induced by ox-LDL,which may be one of its mechanisms in treating ASO. The protective effect of PNS is probably by way of down-regulating the expression of ICAM-1 in endothelial cells and inhibiting the adherence of monocytes to endothelial cells.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2008年第12期1096-1099,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.30572397)
