摘要
目的:观察灯盏细辛注射液(EBI)对大鼠急性心肌梗塞(AMI)后肿瘤坏死因子(TNF-α)及纤溶酶原激活物抑制剂1(PAI-1)、组织纤维蛋白溶酶原激活剂(tPA)的影响。方法:通过结扎大鼠左冠状动脉前降支形成AMI模型,术后24 h后随机分为对照组和实验组,持续1 w每天分别腹腔注射生理盐水、EBI低、中、高剂量,观察EBI对病鼠左心室舒张末期压(LVEDP),升高颈动脉平均动脉压(MAP)、左室最大收缩压(LVPmax)和左室内压最大上升和下降速率(±dp/dt)、TNF-α含量、tPA及PAI-1表达的影响。结果:EBI能显著抑制病鼠TNF-α及PAI-1表达(P<0.05),刺激tPA表达的升高(P<0.05),LVEDP显著降低(P<0.05),显著升高MAP、LVPmax和±dp/dt(P<0.05),作用与剂量呈正相关。结论:EBI可通过抑制TNF-α的过度表达,调节PAI-1与tPA的平衡,减缓血栓形成,改善AMI后心脏的血流动力学。
Objective： To research the effects of Erigeron breviscapus injection（EBI） on TNF-α,PAI-1 and tPA in rats with acute myocardial infarction.Methods： Models of acute myocardial infarction（AMI） were produced by ligation of left anterior descending coronary artery,then rats were randomly divided into control and experimental groups,then respectively gavaged NS,and the low,middle and high dosage of EBI for one week.The cardiac function index and the expression of TNF-α,tPA and PAI-1 were measured.Results： Compared with the NS group,the cardiac function LVEDP,MAP,LVPmax,± dp/dt of AMI rat was improved by EBI in all dosage range,and the expression of TNF-α and PAI-1,LVEDP were decreased（P 0.05）,the expression of tPA,MAP,LVPmax and ± dp/dt were increased obviously（P 0.05） and had a dose-effect relationship.Conclusion： EBI can inhibit the expression of TNF-α and PAI-1,increase the expression of tPA,which can prevent the ongoing thrombopoiesis after AMI and improve the cardiac function.This maybe attributes to the inhibition of the overexpression of TNF-α.
出处
《中药材》
CAS
CSCD
北大核心
2010年第10期1592-1595,共4页
Journal of Chinese Medicinal Materials
基金
广东省社会发展领域科技计划项目(83100)
广东省中医药管理局基金资助项目(2008005)
广东省医院药学研究基金(2009A008)
