摘要
[目的]为常绿水生鸢尾种苗的工厂化生产提供技术依据。[方法]以常绿水生鸢尾组培苗为试材,通过移栽试验研究了组培苗移栽成活率的影响因素。[结果]在基质草炭+砻糠灰+珍珠岩(体积比为7∶2∶1)中组培苗的移栽成活率达90%以上,其余2种基质的移栽成活率都低于60%。移栽后,生根培养基1/2MS+IBA0.5mg/L+NAA0.5mg/L+活性炭(处理A3)中组培苗的平均根数、叶数与主根长均显著优于1/2MS培养基,1/2MS培养基中根茎部无黑色污染状(处理A2)的组培苗显著高于其他2个处理的。处理A2和A3中大、中苗的移栽成活率达95%以上。4月13日与9月9日移栽的组培苗的成活率最高,分别为81%和82%;6月2日移栽的组培苗的成苗时间最短,为36d;11月3日移栽的组培苗成活率低,成苗时间长达120d。[结论]该试验为常绿水生鸢尾的栽培和繁育奠定了基础。
[ Objective ] The purpose of the study was to provide technical basis for the factorial production of water iris seedlings. [ Method ] With the tissue-cultured seedllings of water iris as tested material, the influence factors of transplanting survival rate of tissue-cultured seedlings were studied through transplanting experiment. [ Result] The survival rate of tissue-cultured seedlings transplanted into the substrate peat ＋ rice chaff ash ＋ perlite （Their volume ratio was 7：2：1. ） was above 90% and that of tissue-cultured seedlings transplanted into the other 2 substrates were below 60%. The average root number,leaf number and main root length of transplanted tissue-cultured seedlings from rooting medium 1/2 MS ＋ IBAO. 5 mg/L ＋ NAA O. 5 mg/L ＋ active carbon （ Treatment A3 ） were significantly superior to that of transplanted tissue-cultured seedlings from 1/2 MS media and the transplanted seedlings from 1/2 MS medium and without black pollution on their root crowns （Treatment A2 ） were significantly higher than that from the other 2 treatments. The transplanting survival rates of big and middle tissue-cultured seedlings from Treatment A2 and A3 were above 95%. The survival rates of tissue-cultured seedlings transplanted on Apr. 13th and Sep. 9th were highest and they were 81% and 82% resp. The seedling formation time of tissue-cultured seedlings transplanted on Jun. 2nd was shortest and it was 36 d. The survival rate of tissue-cultured seedlings transplanted after Nov. 3rd was low and their seedling formation time was prolonged to 120 d. [ Conclusion] This experiment laid a foundation for the cultivation and breeding of water iris.
出处
《安徽农业科学》
CAS
北大核心
2009年第34期17251-17253,共3页
Journal of Anhui Agricultural Sciences
基金
江苏省"六大人才高峰"D类资助项目(2008184)
关键词
常绿水生鸢尾
组培苗
成活率
Water iris
Tissue-cultured seedlings
Survival rate
