摘要
以长白山笃斯越桔优良单株的茎尖、茎段为外植体,对其组培快繁技术进行了研究。结果表明:笃斯越桔的幼芽分化适宜的启动培养基为WPM+ZT 2.0mg/L;增殖培养基为改良MS+ZT 0.5mg/L+IBA 0.15mg/L,继代培养30d后的增殖倍数可达5.3倍;适宜的生根培养基为1/4改良MS+IBA 0.5mg/L,培养50d后的生根率可达80%。将笃斯越桔组培苗在纯沙子中练苗20d,将苗移栽至草炭土∶腐殖土为1∶2的基质中,组培苗移栽成活率达78%。
Taking stem or shoot tip of Vacciniunz uliginosurn as the explants, in vitro culture of superior individual of Vaccinium uliginosurn distributing in Changbai Mountain were studied. The results indicated that the media for inducing shoot clusters was WPM＋ZT 2. 0 mg/L; the medium for subculture multiplication culture was modified MS＋ZT 0. 5 mg/L＋IBA 0. 2 mg/L, and the coefficient of multiplication was 5.3 after culture for 30 days;the medium for the rooting was 1/4 MS＋IBA 0. 5 mg/L with rooting rate of over 80% after culture for 50 days. Practices transplanting seedling was to tissue culture in sand 20 days,and then transplanting seedlings to the turfy soil： humus soil 1 ： 2,and survival rate in nursery was up to 78%.
出处
《北方园艺》
CAS
北大核心
2012年第4期113-116,共4页
Northern Horticulture
基金
国家自然科学基金资助项目(30960231)
关键词
笃斯越桔
优良单株
组织培养
快速繁殖
Vaccinium uliginosum
superior individual
in vitro culture
rapid propagation
