摘要
大黄是我国特产的世界著名的传统中药，历代本草均有记载，始载于《神农本草经》，“味苦寒，有毒。主下瘀血、血闭、寒热，破征瘕积聚、留饮宿食，荡涤肠胃，推陈致新，通利水谷，调中化食，安和五脏”。作者已于近期报道了天山大黄发根的诱导方法［１］掌叶大黄Ｒｈｅｕ...
Hairy root culture of the medicinal plant Rheum palmatum L. was established by genetic transformation with Agrobacterium rhizogenes . The effects of various media with different pH on growth of the hairy roots and biosynthesis of free anthraquinones were investigated. The experimental results showed that MS agar medium with pH 5 5～5 8 is suitable for growth of the hairy roots. Dark condition is favourable and 62 5 fold increase in fresh weight was reached within a culture period of 25 days. Auxin (0 1 mg·L -1 IAA) activated the hairy root growth but inhibited the biosynthesis of free anthraquinones. About 28% of the total free anthraquinones was released into the liquid medium from the rhubarb hairy roots.
出处
《药学学报》
CAS
CSCD
北大核心
1998年第11期869-872,共4页
Acta Pharmaceutica Sinica
基金
国家自然科学基金
