摘要
目的：探索用发根培养法生产植物次生代谢物的途径。方法：用发根农杆菌Ａｇｒｏｂａｃｔｅｒｉｕｍｒｈｉｚｏｇｅｎｅｓ９４０２所含Ｒｉ质粒遗传转化药用植物天山大黄以诱导发根用纸电泳法检测发根中冠瘿碱的存在；用高效液相色谱法测定发根中芦荟大黄素、大黄酸、大黄酚、大黄素、大黄素甲醚、大黄酚８甲醚等游离蒽醌化合物的含量。结果：诱导出了天山大黄发根，并建立了发根离体培养系；从发根中检测到甘露碱、农杆碱的存在；定量分析发现，天山大黄发根中含有多种蒽醌类成分，其中大黄酸、芦荟大黄素、大黄素、大黄酚８甲醚的含量分别高于原植物根中的含量。
Objective: To explore the approach to producing plant secondary metabolites using hairy root culture method. Methods: The medicinal plant Rheum wittrochii Lundstr., was genetically with Ri plasmid harbouring in Agrobacterium rhizogenes 9402 strain to induce hairy roots; transformed the opines from the hairy roots were assayed by paper electrophoresis; the contents of free anthroquinones such as aloe emodin, rhein, chrysophanol, emodin, physcion, 8 O methylchrysophanol from the hairy roots were quantitatively determined by HPLC technique. Results: The hairy root was successfully induced and the hairy root culture system was set up; The opines assay was positive for mannopine and agropine; The result of determination showed that there were a lot of anthroquinone constituents in the rhubarb hairy root and the contents of rhein, aloe emodin, emodin or 8 O methylchrysophanol were respectively higher than those in wild plant root. Conclusion: The hairy root cultures of Rheum wittrochii Lundstr. produced similar constitutents as the medicinal site of the wild plant.
出处
《北京医科大学学报》
CAS
CSCD
1998年第5期413-415,共3页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金
国家教委博士点基金
