摘要
背景与目的:已有研究显示表皮生长因子受体(epidermal growth factor receptor,EGFR)及Ⅲ型突变体(epidermal growth factor receptor variant Ⅲ,EGFRv Ⅲ)在一系列恶性肿瘤的发生发展中起重要作用,但EGFR、EGFRvⅢ与食管癌的关系目前尚未明确。本研究探讨EGFR及EGFRvⅢ与食管癌组织发生发展的关系。方法:应用免疫组织化学及蛋白印迹实验(Western Blot)检测66例人食管癌组织及癌旁正常组织EGFR及EGFRvⅢ表达,评价EGFR及EGFRvⅢ在食管癌患者的性别、年龄、TNM分期及病理分级等临床参数组的表达分布。Pearson相关性检验分析EGFR及EGFRvⅢ表达相关性。结果:免疫组化显示EGFR、EGFRvⅢ在肿瘤组织表达的平均灰度净值分别为25.4±3.2、22.5±4.2,在癌旁正常食管组织平均灰度净值分别为5.0±3.5、5.5±3.0,肿瘤组织与正常组织相比差异均有显著性(P<0.000)。Western Blot显示EGFR、EGFRvⅢ在肿瘤组织表达的平均灰度净值分别为1.37±0.41、0.83±0.15,在瘤旁正常食管组织平均灰度净值分别为0.21±0.09、0.08±0.05,肿瘤组织与正常组织相比差异均有显著性(P<0.05)。在性别、年龄、肿瘤大小和生长方式方面EGFR、EGFRvⅢ的表达分布差异均无显著性(P>0.05),而在TNM分期、病理分级和淋巴结转移方面两者差异均有显著性(P<0.05)。免疫组化检测两蛋白表达净灰度值相关系数r为0.701(P<0.0001),Western Blot检测两种蛋白表达相关系数r为0.556(P=0.031)。两种研究方法均提示EGFR与EGFRvⅢ在食管癌中的表达成较好线性正相关性。结论:EGFRvⅢ在食管癌特异性表达,EGFR与EGFRvⅢ在食管癌中的表达呈线性正相关,提示EGFR与EGFRvⅢ与食管癌发生、发展密切相关。
Background and purpose：It has been reported that epidermal growth factor receptor（EGFR）and epidermal growth factor receptor variant Ⅲ（EGFRvⅢ）play important roles in the progression of various cancers.This research was to detect the expression and relation of EGFR and epidermal growth factor receptor variant Ⅲ（EGFRvⅢ）to human esophageal carcinoma.Methods：In 66 human esophageal carcinoma tissues,the expression of EGFR and EGFRvⅢ were detected by immunohistochemistry and western-blot.The expression of EGFR and EGFRvⅢ along with the patients＇ clinicopathologic factors was retrospectively analyzed.Correlation analysis between EGFRvⅢ and EGFR was analyzed by Pearson correlation coefficient.Results：The average gray scale values of EGFR in esophageal carcinoma and normal tissues by immunohistochemistry were 25.4±3.2 and 5.0±3.5,which showed a signifi cant difference（t=5.574,P=0.000）.And the average gray scale values of EGFRvⅢ in esophageal carcinoma and normal tissues were 22.5±4.2 and 5.5±3.0,which also showed a signifi cant difference（t=6.701,P=0.000）.The average gray scale values of EGFR in esophageal carcinoma and normal tissues respectively by western-blot were 1.37±0.41 and 0.21±0.09,which showed a signifi cant difference（t=10.704,P=0.000）And the average gray scale values of EGFRvⅢ respectively were 0.828±0.15 and 0.083±0.049,which had a significant difference（t=9.362,P=0.000）.Significant differences were observed in TNM-stage,lymphatic metastasis and tumor classifi cation in both the expression of EGFR（P0.05）and EGFRvⅢ（P0.05）,and but there were no obvious differences in gender,age,tumor size,growth pattern in both the expression of EGFR（P0.05）and EGFRvⅢ（P0.05）.Correlation analysis showed that there was strong association of the expression between EGFR and EGFRvⅢ both detected by immunohistochemistry（r=0.701,P0.0001）and western-blot respectively（r=0.556,P=0.031）.Conclusion：Our data suggests that EGFRvⅢ is over-expressed in human esophageal carcinoma.Combination of EGFR and EGFRvIII could be useful markers for tumorgenesis and differentiation of human esophageal carcinoma.
出处
《中国癌症杂志》
CAS
CSCD
北大核心
2009年第10期729-734,共6页
China Oncology
基金
国家自然科学基金资助项目(No:30600744)
关键词
表皮生长因子受体
表皮生长因子受体Ⅲ型突变体
食管癌
epidermal growth factor receptor epidermal growth factor receptor variant Ⅲ esophageal carcinoma
