摘要
目的检测表皮生长因子受体Ⅲ型突变体(EGFRvⅢ)在肾透明细胞癌组织中的表达,探讨其与肾透明细胞癌发生、发展的关系。方法采用免疫组织化学染色和图像分析法检测80例肾透明细胞癌和24例正常肾组织中EGFRvⅢ的表达水平,进行统计学分析、比较。结果肾透明细胞癌组织和正常肾组织EGFRvⅢ的表达阳性率分别为46%、8%。通过半定量分析,二者EGFRvⅢ平均灰度值分别为148.49±13.05和155.65±14.86,其差异具有统计学意义(t=2.13,P=0.04);男、女患者EGFRvⅢ的平均灰度值分别为149.01±13.70和147.40±11.76,二者无显著性差异(t=0.54,P=0.59);EGFRvⅢ表达与年龄无显著相关性(r=0.01,P=0.92)。结论EGFR-vIII在肾透明细胞癌组织中存在高表达,与肾透明细胞癌的发生、发展有一定关系。
Objective To detect expression of epidermal growth factor receptor variant Ⅲ （EGFRvⅢ） in human suprarenal epithelioma to explore its relation with the genesis and development of suprarenal epithelioma. Methods Immunohistochemistry and pathologic image analysis were used to semiquantitatively detect the expression of EGFRv Ⅲ protein in eighty human suprarenal epithelioma tissues and twenty-four normal renal tissues. Results Positive expression rate of EGFRvⅢ was 46% in human suprarenal epithelioma tissues and 8% in normal renal tissues. Their average gray scale values were 148.49 ± 13.05 and 155.65 ± 14.86, respectively, which showed a significant difference （t =2.13, P=0.04）. There was no obvious difference between males and females （149.01 ± 13.70 and 147.40 ± 11.76, t = 0.54, P = 0.59）. No significant association was observed between EGFRvⅢ expression and age （r =0.01, P = 0.92）. Conclusion Many human suprarenal epithelioma tissues express EGFRvⅢ . EGFRvⅢ may play certain role in the genesis and development of human suprarenal epithelioma.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2008年第4期441-442,449,共3页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
国家自然科学基金资助项目(No.30600744)
西安交通大学医学院第一附属医院青年创新基金(No.2006YK9)
