摘要
目的：建立HPLC测定大鼠血浆丹参素的浓度,比较丹参素钠及丹参注射液在大鼠体内的药动学特点,评价丹参注射液中其他成分对丹参素药动学的影响。方法：大鼠分别iv给予丹参素钠或丹参注射液（丹参素30 mg.kg-1）,颈动脉取血,分离血浆,高氯酸萃取后进行HPLC分析,测定血浆丹参素浓度。DAS2.0软件处理数据。结果：丹参素在0.5~80.0 mg.L-1内线性良好,最低检测浓度为0.2 mg.L-1。大鼠静脉给予丹参素钠和丹参注射液后的丹参素浓度-时间曲线均符合二室模型,两药t1/2α,AUC,CL等参数有显著性差异。结论：丹参注射液中共存成分可影响丹参素的体内过程,加快其分布和消除。
Objective： To develop a HPLC method for determination of the concentration of Danshensu in rat plasma and undertake comparative pharmacokinetic study of sodium danshensu and Salvia miltiorrhiza injection in rat as well as to assess the effect of other components of Salvia miltiorrhiza injection on the pharmacokinetics of Danshensu. Method： Rats received an iv infusion of sodium Danshensu or S. miltiorrhiza injection（ equal to Danshensu 30 mg· kg^-1）. Blood samples were collected from carotid artery. Plasma concentration of Danshensu extracted with perchloric acid was measured. The pharmacokinetic parameters were calculated with DAS2. 0 software. Result： A good linear relationship of Danshensu was obtained from the range of 0. 5 to 80. 0 mg · L^- 1, and the lowest limit of determination was 0. 2 mg · L^- 1. The plasma concentration time curves of Danshensu were best fitted with two-compartment models for Danshensu itself and for Salvia miltiorrhiza injection as well. The pharmacokinetic parameters such as t1/2, AUC, CL had significant differences. Conclusion： The concomitant components in Salvia miltiorrhiza injection influence the pharmacokinetic properties of Danshensu and speed up its disposition and elimination.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第22期2943-2945,共3页
China Journal of Chinese Materia Medica
基金
安徽省高校省级自然科学研究项目(KJ2008B084)
关键词
丹参素钠
丹参注射液
药动学
液相色谱
sodium Danshensu Salvia miltiorrhiza iniection oharmacokinetics HPLC
