摘要
目的:建立家兔血液中七叶皂苷钠的测定方法。方法:应用酶联免疫吸附分析(ELISA)方法,测定正常家兔与脑缺血再灌注模型家兔静脉注射β-七叶皂苷钠(5mg/kg)后的血药浓度,并对其药动学参数进行分析。结果:正常家兔与模型家兔静脉注射β-七叶皂苷钠后,主要药动学参数t1/2α分别为(0.343±0.061)、(0.854±0.079)h,t1/2β分别为(23.325±10.36)、(34.283±13.74)h,CL分别为(1.600±1.206)、(0.718±0.428)L/h,Vd分别为(53.827±36.42)、(14.799±10.15)L,AUC0-24h分别为(2.919±0.981)、(26.417±9.207)μg(/h.L),AUC0-∞分别为(3.126±1.253)、(27.848±8.745)μg(/h.L),cmax分别为(4.126±0.927)、(7.905±1.054)mg/L。结论:β-七叶皂苷钠在脑缺血再灌注模型大鼠体内的消除较正常大鼠慢,在体内停留的时间较长。提示β-七叶皂苷钠在临床对症治疗时应考虑其药动学特点。
OBJECTIVE： To establish a method for the concentration determination of β-aescin sodium in blood of rabbits. METHODS： ELISA method was adopted. The blood concentration of β-aescin sodium in normal rabbits and cerebral ischemia-reperfusion rabbits were determined after i.v. injection of β-aescin（5 mg/kg）. The pharmacokinetic parameters were analyzed. RESULTS： After i.v. injection ofβ-aescin sodium, main pharmacokinetic parameters of normal rabbits vs. model rabbits were as follows： t1/2a were （0.343 ± 0.061） h vs.（0.854 ± 0.079） h;t1/2β were （23.325 ± 10.36） h vs. （34.283 ± 13.74） h; CL were （1.600 ± 1.206） L/h vs. （0.718 ± 0.428） L/h; Vd were （53.827 ± 36.42） L vs.（14.799 ± 10.15） L; AUC0-24b were （2.919 ± 0.981） μg/（h-L） vs.（26.417 ± 9.207） μg/（h-L） ; AUC0-ω were （3.126 ± 1.253） μg/（h-L） vs.（27.848 ± 8.745） μg/（h-L） ;Cmax were（4.126 ± 0.927） mg/L vs. （7.905 ± 1.054） mg/L. CONCLUSION： The elimination of β-aescin sodium in cerebral ischemia-reperfusion rats is slower than in normal rats, remain inside the body for a long time. The pharmacokinetic characteristics ofβ-aescin should be considered in the clinical symptomatic treatment.
出处
《中国药房》
CAS
CSCD
2013年第15期1362-1364,共3页
China Pharmacy
