摘要
目的:观察金荞麦抗肿瘤提取物(FR)、苦参抗肿瘤提取物(MA)对肺癌PG细胞-血管内皮(HU-VEC)黏附作用的影响,并探讨其抗肿瘤转移机制。方法:运用MTT检测FR/MA单药及联合用药对高转移肺癌PG细胞增殖的作用,以荧光标记PG-HUVEC黏附模型考察FR/MA单药及联合用药抗肿瘤细胞的黏附能力,用Western blotting、流式细胞仪检测FR/MA单药及联合用药对黏附分子表达的影响。结果:FR/MA单药及联合用药均剂量依赖性抑制PG细胞增殖(P<0.05);在一定浓度范围内,FR/MA单药及联合用药显著抑制PG-HU-VEC相互黏附能力并有剂量依赖性(P<0.01);FR/MA联合用药显著抑制黏附分子CD44、CD49、ICAM-1及E-selectin的表达(P<0.01)。结论:FR/MA具有抗PG-HUVEC相互黏附作用;降低细胞表面黏附分子的表达,抑制肿瘤细胞在血管壁着床可能是其抗肿瘤转移的作用机制之一。
Objective：To study the effect of Fagopyrurn cymosum Meisn extract （FR） and Matrine （MA） between high metastasis lung cancer cell line（PG） and human umbilical vein endothelial cell（HUVEC） and its mechanism. Methods： MTT experiment were used to evaluate PG cell growth; the PG - HUVEV adhesion model labeled with a fluorescent dye were usede to evulate the effect of FR/MA/FR ＋ MA on the adhesion ability; Western - blotting assay and flow cytometry were introduced to measure the effect of FR ＋ MA on the expression of adhesion molecules. Result： Both FR/MA and FR ＋ MA inhibited the PG cell growth（P 〈0.05）. FR/MA and FR ＋MA are also able to significantly reduce the adhesion activity between tumor cells and endothelial cells to some extent（P 〈 0.01 ）, FR ＋ MA can obviously inhabit the expression of adhesion molecules CD44 ,CD49 ,ICAM - 1 and E - selectin（ P 〈0.01 ）. Conclusion：FR/MA can reduce the adhesion ability between PG tumor cells and endothelial cells, one of the possible mechanisms may be inhabit the expression of adhesion molecules and preventing tumor cells to adhere to vessel wall.
出处
《中华中医药学刊》
CAS
2009年第11期2374-2376,共3页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省中医药科技计划项目资助(2005A109)
