摘要
目的：探讨氧自由基在脑缺血、再灌流损伤中的作用，为临床进行抗氧化治疗提供依据。方法：用Ｗｉｓｔａｒ大鼠制成左侧大脑中动脉缺血、再灌流模型，观察生化代谢、自由基代谢、组织和超微结构改变。结果：再灌流组较缺血组血清ＣＰＫ增高、ＭＤＡ升高，再灌流组脑组织中ＭＤＡ升高，血清中ＳＯＤ活力下降，再灌流组脑组织结构和超微结构破坏更加严重，应用自由基清除剂后血清中ＣＰＫ降低，ＭＤＡ含量降低，ＳＯＤ活性升高，组织中ＭＤＡ含量亦降低，超微结构较前好转。结论：自由基反应增强是脑组织缺血、再灌流损伤的主要原因之一，应用自由基清除剂可减轻对脑组织的损害。
Objective: To investigate the relationship between cerebral ischemia/reperfusion injury and oxygen free radical.And to provide the role of the free radical scavengation therapy in ischemic cerebrovascular disease.Method:Left middle cerebral artery(MCA) ischemia/reperfusion models of Wistar rats were made. The changes of biochemical metobolism and oxygen free radicals in serum and cerebral tissue in periods of ischemia and reperfusion were observed. At the same time,the protective effect of mannital and VitC were assessed.Result:The serum level of creative phosphokinase(CPK) and malon dialdehyde(MDA) was higher,but superoxide dismutase(SOD) activity was lower during reperfusion than in the ischemic period.The tissue structure and ultrastructure were damaged more seriously in reperfution period. After treated by mannital and VitC, the level of CPK and MDA in serum was lower, but SOD activity rasied, and the ultrastructure was better. Conclusion:Increasing of oxygen free radical reaction is an important reason of ischemia/reperfusion injury. Free radical scavengers could decrease the injury. Author′s address\ Department of Neurology,Second Affiliated Hospital, Institute of Neurology Lanzhou Medical College,Lanzhou,730030
出处
《中国康复医学杂志》
CAS
CSCD
1998年第6期254-257,共4页
Chinese Journal of Rehabilitation Medicine
关键词
脑缺血
再灌注损伤
氧自由基
清除剂
Ischemia
Reperfusion injury
Oxygen free radical
Scavengers
