摘要
观察甘露醇对脑缺血鼠脑组织羟自由基的清除作用。方法采用线栓法制成大脑中动脉脑缺血模型，利用高压液相色谱技术测定脑组织中羟自由基的含量。结果脑缺血组鼠脑皮层及纹状体内２，３－二羟基苯甲酸（２，３－ＤＨＢＡ）、２，５－ＤＨＢＡ的水平较对照组增高３～４倍。甘露醇能有效地降低局灶性缺血脑皮层及纹状体２，３－ＤＨＢＡ及２，５－ＤＨＢＡ的水平，改善缺血后神经功能损害，且甘露醇持续慢滴组较快滴组效果明显。甘露醇快速滴注组能减轻缺血脑组织的水肿，而甘露醇慢滴组作用不明显。结论甘露醇的脑保护作用不完全与脱水降颅压有关。
Objective It was designed to find out whether the lipid peroxidation caused by free radicals might take part in causing the damage of cerebral ischemic neurons. Method In this study, the focal cerebral ischemia models of rats were made with thread embolism of the middle cerebral artery(MCA). The hydroxyl radical(OH ·) could be trapped by salicylate to form 2,3 and/or 2,5 dihydroxybenzoic acid (DHBA) which were readily measured with high performance liquid chromography (HPLC). Results We found that the levels of 2,3 and 2,5 DHBA formation were increased for about 3 4 folds in the striatum and cerebral cortices of the rats after MACO, respectively. Exidently mannitol could attenuate the increased 2,3 and 2,5 DHBA levels in the striatum and cerebral cortices following focal ischemia in the rats and improve the damage of the neuronal function. However, quick dripping injections of mannitol solution could reduce the edematous areas in the ischemic brain tissues. slow dripping injections of mannitol solution showed no significant effect on reducing the edematous areas of the rat brains. Conclusion These results suggested that mannitol injections could reduce free radicals production and this protective activity would probably and incompletely be linked with its dehydration effect.
出处
《中华神经科杂志》
CAS
CSCD
1997年第3期161-164,共4页
Chinese Journal of Neurology
关键词
脑缺血
甘露醇
药物疗法
Cerebral ischemia Mannitol Hydroxyl radical
