摘要
对中国花生（ＡｒａｃｈｉｓｈｙｐｏｇａｅａＬ．）５种不同类型的４６个栽培品种的种子蛋白质进行电泳分析。ＳＤＳＰＡＧＥ和ＩＥＦＳＤＳＰＡＧＥ（双向电泳）的结果显示，不同品种的花生种子蛋白多肽组成存在４种类型。与初步纯化的花生球蛋白及伴花生球蛋白的ＳＤＳＰＡＧＥ结果比较，花生种子蛋白组成的主要差异在于花生球蛋白亚基组成的不同，其中类型Ⅰ花生球蛋白主要含有４１ｋＤ、３８５ｋＤ和２个１８ｋＤ亚基，类型Ⅱ主要含４１ｋＤ、３８５ｋＤ、３７５ｋＤ和３个１８ｋＤ亚基，类型Ⅲ主要含４１ｋＤ、３８．５ｋＤ、３６．５ｋＤ和３个１８ｋＤ亚基，类型Ⅳ主要含４１ｋＤ、３８．５ｋＤ、３７５ｋＤ、３６．５ｋＤ和３个１８ｋＤ亚基。不同品种的伴花生球蛋白多肽组成基本一致。对不同类型８个品种种子蛋白的氨基酸组成进行测定，发现类型Ⅰ的含硫氨基酸含量较高。
Seed polypeptides from 46 cultivars of peanut (Arachis hypogaea L.) were compared by SDSPAGE and twodimensional polyacrylamide gel electrophoresis. Arachin, the major seed storage protein of peanut, showed polymorphism. There were four types of arachin polypeptide patterns. Type Ⅰ consisted of only four major subunits:41 kD,38.5 kD and two of the 18 kD subunits. Type Ⅱ had six major subunits:41 kD,38.5 kD,37.5 kD and three of the 18 kD subunits. Type Ⅲ consisted of 41 kD, 38.5 kD,36.5 kD and three of the 18 kD subunits. And Type Ⅳ consisted of seven major subunits:41 kD,38.5 kD,37.5 kD,36.5 kD and three of the 18 kD subunits. The compositions of conarachin in different cultivars were similar. Amino acid composition analysis of seed protein in 8 peanut cultivars showed that Type Ⅰ was rich in methionine and cystine.
基金
广东省自然科学基金
