摘要
利用氨基硅烷偶联剂KH-792对醋酸纤维素(CA)表面进行改性,同时研究了表面改性后的CA对脂肪酶的固定效果。红外光谱测定了硅烷剂以化学键的形式结合在CA表面,使CA表面带有了氨基活性基团。有机元素分析表明,硅烷剂的接枝量为11.66%。当改性时间为24h,硅烷剂浓度为6%时,酶固定量和活性收率最高,分别为25.8mg/g和43.3%。
The modification of acetyl cellulose （CA） with amino-silane coupling agent KH-792 was studied. Infrared spectroscopy proved that the silane agent was grafted on the CA surface by chemical bonds and the elemental analysis gave the amount of amino-group on the CA surface. At the same time, the study of immobilizing enzyme on the modified CA was done. The most amount of immobilized Lipase enzyme and the relative activity were 25.8mg/g and 43.3 percent when the modified time was 24h and silane concentration was 6%.
出处
《化工新型材料》
CAS
CSCD
北大核心
2009年第10期60-62,共3页
New Chemical Materials
基金
国家自然科学基金(20676125)
国家863计划基金(2008AA062330)
关键词
醋酸纤维素
硅烷偶联剂
表面改性
固定酶
acetyl cellulose, silane coupling agent, surface modification, enzyme immobilization
