摘要
以细菌纤维素为载体,采用吸附-交联的方法将海藻糖合酶固定化,在最适固定化条件下,15℃吸附20h,然后与6%戊二醛在15℃交联20h。实验发现,与游离酶相比,固定化酶的最适pH值向碱性方向移动0.4,为pH7.4,最适作用温度为45℃,比游离海藻糖合酶提高10℃,酸碱稳定性、热稳定性均有较大提高;重复使用6次后,酶剩余活力保持在87%左右,有较好的操作稳定性和重复使用稳定性。
The trehalose synthase was immobilized on bacterial cellulose by adsorption and crosslinking. The optimum conditions for immobi- lization were investigated and stated as 20p.l trehalose synthase was adsorbed with lg dry bacterial cellulose at pH 7.0 and 15℃ for 20h, fol- lowed by being treated with 6% glutaraldehyde at 15℃ for 20 h. Some properties of the immobilized enzyme were compared with those of the nature enzyme. The optimum pH of the immobilized enzyme was 7.4, which was 0.4 higher than that of the nature enzyme. Besides, its optimum reaction temperature was 45℃, which was 10℃ higher than that of the nature enzyme. The stabilities of trehalose synthase on enduring pH changing, heat treatment and multiple utilization were obviously improved after immobilization.
出处
《中国酿造》
CAS
北大核心
2006年第9期19-23,共5页
China Brewing
基金
山东省优秀中青年科学家基金项目(BS0384)
关键词
海藻糖合酶
细菌纤维素
固定化酶
trehalose synthase
bacterial cellulose
immobilization
