摘要
目的建立HPLC-ELSD测定轻身减肥片中黄芪甲苷含量的方法。方法选用C_(18)色谱柱(4.6mm×250mm,5μm),流动相为乙腈-水(35:65)蒸发温度120℃,雾化温度80℃,载气流速1.6 mL/min,柱温35℃。结果黄芪甲苷在1.03～8.24 μg范围内呈良好的线性关系,r = 0.999 9,平均加样回收率为99.35%,RSD = 1.36% (n = 6)。结论所建立的方法简便、准确,重复性好,精密度高,可用于轻身减肥片的质量控制。
Objective To establish a HPLC-ELSD method for determining the content of astragaloside Ⅳ in Qingshen dianfei Tablets. Methods Stationary phase was C18 column [4.6 mm × 250 mm, 5μm）, mobile phase was acetonitrice-water （35 ： 65）. The evaporation temperature was 120 ℃, the pulverization temperature was 80 ℃, the flow rate was 1.6 mL/min and column temperature was 35 ℃. Results The standard curve was linear within the range of 1.03-8.24 μg, r =0.9999. The average recovery was 99.35% with RSD = 1.36% （n = 6）. Conclusion The established method is simple and accurate, with good reproducibility and high precision, and suitable for the determination of astragaloside Ⅳ in Qingshen dianfei Tablets.
出处
《中国中医药信息杂志》
CAS
CSCD
2009年第11期46-47,共2页
Chinese Journal of Information on Traditional Chinese Medicine
