摘要
目的 :运用反相高效液相色谱法测定轻身减肥片中大黄素及大黄酚的含量。方法 :采用ZorbaxC1 8柱 ,甲醛 - 0 1%磷酸溶液 (80∶2 0 )为流动相 ,检测波长为 2 5 4nm。结果 :大黄素的回收率为 95 8% ,RSD =1 86 % ;大黄酚为 95 7% ,RSD =1 93%(n =5 )。结论 :本法简便、快速、重现性好 ,可作为制剂的定量分析方法。
Objective:To determine the contents of Emodin and Chrysophanol in Qingshenjianfei Tablets by RP HPLC.Methods:column in this methods Zorbax C 18 ∶mobile phase:methanol-0 1% phosphoric acid(80∶20);detection wavelenght:254nm.Results:Recovery and RSD of Emodin and chrysophanol were 95 8%,1 86%;95 7% and 1 93%( n =5)respectively.Conclusion:This method is simple and quick,reproducible,and can be used for the quantitative analysis.
出处
《中国实验方剂学杂志》
CAS
2002年第5期4-6,共3页
Chinese Journal of Experimental Traditional Medical Formulae
