摘要
目的：观测β－葡萄糖醛酸酶（β－Ｇｌｕｃｕｒｏｎｉｄａｓｅ，β－Ｇ）在人体胰腺导管上皮细胞与胰腺癌细胞中的定位，探索良、恶两种细胞内β－Ｇ的差异。方法：采用低温包埋，紫外线照射，超薄切片，胶体金标记，免疫电镜方法。结果：β－Ｇ定位于内质网和溶酶体内，同时观察到胰腺癌细胞中标记β－Ｇ的金颗粒数目明显多于胰腺导管上皮细胞中金颗粒数目。结论：癌细胞分泌β－Ｇ增多，对胰腺癌的早期发现、早期诊断有一定意义。
Objective:This paper was to localize β Glucuronidase (β G) in duct epithelial cells and carcinous cells of pancreas,and to explore the difference of β G between benign and malignant cells. Methods:we used low temperature embedding, ultraviolet ray shining, ultraslice, colloid gold labelling,and immune electron microscopic technique. Results:we localized β G in endoplasmic reticulum and lysosome in duct epithelial cells and carcinous cells of pancreas. The number of gold granules labelled with β G was more in carcinous cells than that in duct epithelial cells. Conclusions:The increase of β G secretion in carcinous cells may be of significance for early discovery and early diagnosis of pancreatic carcinoma.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1998年第5期451-453,共3页
Journal of China Medical University
关键词
胰腺肿瘤
胰腺导管
上皮细胞
葡萄糖醛酸酶
β Glucuronidase
Colloid gold
immunol electron microscope
duct epithelial cells of pancreas
carcinous cells of pancreas
