摘要
目的:研究人体乳腺导管上皮细胞内β-葡糖醛酸酶(β-Glu)超微结构水平的定位。方法:采用抗β-Glu抗体,胶体金探针及免疫电镜技术与方法。结果:标记β-Glu的金颗粒定位于内质网和溶酶体内。结论:为今后研究乳腺各种疾病和肿瘤β-Glu活性的变化,建立了一个正常的形态学研究模型。
Objective：To study the altrastructure localization of β-glucuronidase （β-Glu） in the human breast duct epithelial cells. Methods：Anti-β-Glu antibody, colloid gold probe, and immunoelectron microscope were used. Results：Golden grains labeled β-Glu were localized in the endoplasmic reticulum and lysosome. Conclusion：Successful localization of β-Glu in the endoplasmic reticulum and lysosome in the breast duct epithelial cells could be used as the normal morphologic model to study benign breast diseases and breast neoplasms.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2007年第5期562-563,共2页
Journal of China Medical University
关键词
β-葡糖醛酸酶
胶体金
免疫电镜
内质网
溶酶体
β-glucuronidase
colloid gold
immunoelectron microscope
endoplasmic reticulum
lysosorne
