摘要
[目的]建立采用毛细管电泳-电化学法分析干红枣中维生素含量的测定方法。[方法]采用自组装的毛细管电泳电化学检测系统,对干红枣中Vc、VP、VE的含量进行分离检测,对测定时不同的工作电位、分离高压、pH值和有机溶剂进行最佳选择。[结果]在选用pH值为8.0的硼砂-硼酸缓冲液,CTAB为0.5mmol/L、分离高压为20kV、检测电位为0.8V的测定条件下,Vc的线性范围为0.10～5.10mg/L,r=0.9991,检出限为1.37mg/L;VP的线性范围为1.30～17.70mg/L,r=0.9996,检出限为1.23mg/L;VE的线性范围为0.21～9.60mg/L,r=0.9990,检出限为0.27mg/L。测得的干红枣Vc、VP、VE的含量分别为424.64、412.16、312.39mg/100g,平均回收率分别为97.7%、98.1%和96.7%。[结论]毛细管电泳-电化学测定法快速、灵敏度高、选择性强,是适合干红枣维生素含量快速检测的方法。
[ Objective ] The study aimed to establish the determination method for vitamin contents in dried red jujube by capillary eleetrophoresis with electrochemical detection. [ Method] The self-made capillary eleetrophoresis with electrochemical detection system was used to separate and determine Vc, VP, VE contents in dried red jujube and the different working potentials, separation voltages, pH values and organic solvents in the determination were screened optimally. [ Result] Under the determination condition of selecting berax-boraeie acid buffer solution with pH of 8.0, CTAB of 0.5 mmol/L, separation voltage of 20 kV, detecting potential of 0.8 V, the linear range of Ve, VP, VE were 0.10 - 5.10, 1.30 - 17.70 and 0.21 - 9.60 mg/L resp. , their correlation coefficients were 0.999 1,0.999 6 and 0.999 0 and their detection limits were 1.37, 1.23 and 0.27 mg/L. The determined Vc, VP, VE contents in dried red jujube were 424.64, 412.16 and 312.39 mg/100 g resp. , with the mean recovery rate being 97.7%, 98.1% and 96.7% resp. [ Conclusion] The determination method of capillary electrophoresis with electrochemical detection system had characters of rapid speed, high sensitivity and strong selection, which was a rapid determining method suitable to detect the vitamin contents in dried red jujube.
出处
《安徽农业科学》
CAS
北大核心
2009年第30期14554-14555,14557,共3页
Journal of Anhui Agricultural Sciences
