摘要
建立梯度洗脱反相高效液相色谱法同时检测果醋中VC、VB1、VB2、VB3、VB6、VB1 2、叶酸和烟酰胺8种水溶性维生素的分析方法。考察柱温、缓冲液浓度、pH值以及梯度洗脱程序对各组分分离的影响。最终采用0.1mol/L醋酸钠溶液(pH4.25)和甲醇作为流动相进行梯度洗脱,流速为1.00mL/min,柱温为30℃,8种水溶性维生素在14.2min内达到基线分离;紫外检测波长为266nm和280nm;线性范围:VB2、叶酸为0.05～12.5μg/mL;VC、VB1、VB3、烟酰胺为0.1～25μg/mL;VB6、VB12为0.15～37.5μg/mL,相关系数均在0.999以上,其最低检出限为0.47～3.46ng/mL。该法被成功地用于定量果醋中的维生素,平均加标回收率为92.4%～105.2%,相对标准偏差为0.08%～1.54%。
A RP-HPLC gradient elution method was developed for the simultaneous determination of eight water-soluble vitamins, including ascorbic acid, thiamine, riboflavin, nicotinic acid, pyridoxine, cyanocobalamin, folic acid and nicotinamide in fruit vinegar. The effects of temperature, buffer solution concentration, pH value and gradient elution procedure on chromato- graphic separation were studied. The mobile phase was a mixture of 0.1 mol/L sodium acetate solution （pH 4.25） and methanol at a flow rate of 1.00 mL/min. The column temperature was set as 30 ~C. Baseline separation was achieved for 8 water-soluble vitamins within 14.2 min. The detection was carried out at 266 nm and 280 nm. The method offered excellent linearity over a range of 0.05-- 12.5 lag/mL for riboflavin and folic acid, 0.1 --25 ~t g/mL for ascorbic acid, thiamine, nicotinic acid and nicotinamide, 0.15 -- 37.5 /a g/mL for pyridoxine and cyanocobalamin, with correlation coefficients （r ~ 0.999）. The detection limits were in the range of 0.47--3.46 ng/mL This method has been successfully applied to assay vitamins in fruit vinegar. The average spike recovery varied from 92.4% to 105.2% with RSD in the range of 0.08% -- 1.54% （n =3）.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第12期181-184,共4页
Food Science
基金
西北农林科技大学引进人才专项(01140414)
