摘要
目的研究氢气饱和生理盐水对肺型氧中毒的影响。方法Sprague—Dawley大鼠持续暴露于浓度大于98％的氧（0．25MPa，5h）中，持续小流量通风，制备肺型氧中毒模型。动物分组：常压空气对照组、生理盐水模型组、氢气饱和生理盐水模型组。应用HE染色观察肺部形态学变化，检测支气管肺泡灌洗液（bronchoalveolar lavage fluid，BALF）中总蛋白含量以及总细胞计数。结果与生理盐水组相比，氢气饱和生理盐水明显减轻高压氧造成的肺损伤，减轻肺组织病理学损伤，减少炎细胞浸润，减少BALF中蛋白含量。结论氢气饱和生理盐水可以减轻肺型氧中毒的损伤。
Objective To investigate the effect of saturated hydrogen saline toxicity induced by hyperbaric oxygen exposure. Methods Sprague-Dawley （SD） on pulmonary oxygen rats were exposed to hyperbaric environment with 98% oxygen concentration at 0. 25 MPa for 5 hours to develop the model of pulmonary oxygen toxicity. The animals were randomly divided into 3 groups：the normal air control group, the saline group, and the saturated hydrogen saline group. Hematoxylin and eosin （H＆E） staining were used to observe morphological changes in the lung and measure the concentration of protein and total cell counts in bronchoalveolar lavage fluid（BALF）. Results Microscopy revealed that pulmonary impairment in the saturated hydrogen saline group was obviously less severe than that of the saline group. Saturated hydrogen saline could markedly alleviate pathological lesion in the lung tissue, ease inflammatory cell infiltration and decrease total cell and protein concentrations in BALF. Conclusions Saturated hydrogen saline could alleviate pulmonary lesion induced by hyperbaric oxygen exposure.
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
2009年第4期197-199,共3页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
基金
国家自然科学基金课题（30500579）
关键词
氢气饱和生理盐水
高压氧
肺损伤
肺型氧中毒
Saturated hydrogen saline
Hyperbaric oxygen
Lung lesion
Pulmonary oxygen toxicity
