摘要
目的探讨核因子（NF）-κB在大鼠肺型氧中毒发病中的作用。方法32只SD大鼠随机分为正常对照组及230 kPa高压氧暴露2、6、10h组。观察肺组织病理变化以及NF-κB和肿瘤坏死因子α（TNF-α）在肺组织中含量的变化。结果（1）病理检查发现肺组织在高压氧暴露6h组出现炎症改变，10h组肺损伤加重。（2）肺组织细胞核中NF-κB P65含量在高压氧暴露2h组中明显增高，6h组中达高峰，10h组中下降但仍高于正常对照组。（3）肺组织TNF-α mRNA表达及肺匀浆中TNF-α浓度在高压氧暴露6h组中明显增高，10h组中继续增高。结论高压氧可以通过活化NF-κB诱导TNF-α／高表达从而介导氧中毒的肺损伤。
Objective To investigate effects of nuclear factor-kappa B （NF-κB） on pulmonary, oxygen toxicity in rats. Methods Thirty-two SD rats were randomly divided into 4 groups： normal control group; 2, 6 and 10 h of 230 kPa hyperbaric oxygen （HBO） exposure groups. Pathological change, the contents of NF-κB and TNF-α in lung tissue were observed. Results （1） Inflammation of lung was found after 6 h of HBO oxygen exposure and aggravated after 10 h of HBO exposure. （2） The contents of NF-κB P65 in lung tissue nucleus increased significantly after 2 h and reached the peak value after 6 h of HBO exposure. It declined after 10 h of HBO exposure but was still higher than the content of normal control group. （3） The expression TNF-α mRNA in lung tissue and content of TNF-α in lung homogenate increased significantly after 6 h and continued to increase after 10 h of HBO exposure. Conclusions HBO may activate NF-κB to elevate expression of TNF-α, which may lead to oxygen toxicity lung injury.
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
2008年第3期156-159,共4页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
基金
基金项目：总后卫生部科研项目（04-J008）,海后重点科研课题（07-3301）
