摘要
目的:探讨P物质(substanceP,SP)在变应性鼻炎中对一氧化氮(nitricoxide,NO)的影响。方法:以卵清蛋白建立变应性鼻炎豚鼠模型。然后用SP滴鼻(1次/d)激发变应性鼻炎各组1、2、4、8d,并与正常组对照,观察其症状和体征。同时观察鼻黏膜病理学变化,并采用硝酸还原酶法测定各组豚鼠的鼻腔灌洗液和血清中NO3-/NO2-的含量确定NO的浓度。结果:SP激发能诱发正常的豚鼠出现相似的变应性鼻炎症状,并能加重模型组豚鼠变应性鼻炎症状和鼻黏膜炎症(P<0.01)。模型组SP激发后鼻腔灌洗液和血清中NO含量明显增加,并且随着激发次数的增加,NO有逐渐增高的趋势(P<0.05)。结论:在变应性鼻炎中,SP能促进NO在鼻黏膜的产生,引发和加重变应性鼻炎的炎症反应。
Objective:To study the effects of substance P(SP) on NO in allergic rhinitis(AR). Methods: An AR model was established in healthy guinea pigs by using ovalbumin, where the AR animals were intranasally challenged with SP,and the symptoms and signs were observed and compared with those of normal controls. Meanwhile, histopathologic changes of nasal mucosa were observed with H-E staining, and the density of NO 3-/NO 2- in the serum and nasal lavage fluid was determined by nitrate reductase method to assay the content of NO. Results: SP stimulated typical symptoms in normal group similar to those in AR group, and aggravated the symptoms and histopathologic inflammatory reactions in AR groups(P<0.01). The density of NO in the serum and nasal lavage fluid of AR groups increased significantly after challenge with SP, and NO tended to increase with the increase of stimulation time(P<0.05). Conclusion: SP can promote the production of NO,induce and aggravate allergic inflammation.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2005年第6期654-656,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金(30271406)
上海市卫生系统百名跨世纪优秀学科带头人培养基金(98BR003).
关键词
变应性鼻炎
P物质
一氧化氮
allergic rhinitis
substance P
nitric oxide
