摘要
目的:研究人乳头瘤病毒16型(HPV16)DNA阳性乳腺良、恶性病变组织中E6和E7蛋白的表达情况。方法:以经PCR筛选证实的HPV16DNA阳性的56例乳癌和41例乳腺良性病变组织为研究对象,采用Westernblotting方法检测HPV16E6和E7蛋白。结果:56例HPV16DNA阳性乳癌组织中,HPV16E6、E7和E6/E7共表达阳性率分别为67.9%(38/56)、75.0%(42/56)和57.1%(32/56);41例HPV16DNA阳性乳腺良性病变组织中,HPV16E6、E7和E6/E7共表达阳性率分别为36.6%(15/41)、43.9%(18/41)和24.4%(10/41)。HPV16DNA阳性乳癌组织中E6、E7和E6/E7共表达阳性率均高于乳腺良性病变组织(χ2分别为9.34,9.70,10.34,P均<0.01)。HPV16DNA阳性乳癌组织中E6、E7和E6/E7共表达阳性率与患者年龄和肿瘤组织学类型无关(P均>0.05),但与淋巴结转移有关(χ2分别为3.88,15.86,9.34,P均<0.05)。结论:HPV16E6和E7蛋白在乳癌发生发展中具有重要作用,并与乳癌淋巴结转移有关。
Aim：To investigate the expressions of human papillomavirus 16 type（HPV16） E6 and E7 proteins in HPV16 DNA-positive breast benign and malignant lesions.Methods：A total of 56 HPV16 DNA-positive breast carcinomatissue and 41 breast benign lesions confirmed by PCR were used to detect HPV16 E6 and E7 proteins using Western blotting assay.Results：In 56 HPV16 DNA-positive breast carcinoma tissue,the positive rates of HPV16 E6,E7,and E6/E7 co-expression were 67.9%（38/56）,75.0%（42/56）,and 57.1%（32/56）,respectively,all higher than those of 36.6% （ 15/41 ） , 43.9% （ 18/41 ） , and 24.4% （ 10/41 ） , respectively, in 41 HPV16 DNA-positive breast benign lesions（χ^2 was 9.34,9.70,10. 34, respectively, P 〈 0.01 ）. The HPVI6 E6, E7, and E6/E7 co-expression were associated with lymph node metastasis of patients with breast carcinoma （χ^2 was 3.88,15.86,9.34, respectively, P 〈 0.05 ） , but not with age or histological classification （P 〉0.05 ）. Conelusion：HPV16 E6 and E7 proteins may play an important role in the carcinogenesis and progression, and were associated with lymph node metastasis of breast carcinoma.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2009年第4期717-719,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
卫生部科研基金资助项目WKJ2007-2-022
河南省高校杰出科研人才创新工程资助基金项目2007KYCX013
