摘要
背景与目的中国河南省安阳地区是食管癌高发区,有研究发现人乳头状瘤病毒(humanpapillomavirus,HPV)的感染是安阳地区食管癌的重要发病因素。本文研究HPV16型在中国北方不同地区食管癌患者中的感染率及其表达水平,以进一步明确HPV16与食管癌发病的相关性。方法用地高辛标记的HPV16E6探针做原位杂交(insituhybridization,ISH),检测河南省安阳食管癌高发区的食管癌患者(43例)、北京肿瘤医院散发食管癌患者(43例)和内蒙古自治区蒙古族食管癌患者(33例)的食管癌组织中HPV16的感染情况。结果ISH检查结果显示安阳、北京、内蒙古3个地区食管癌患者HPV16感染率分别为81.4%(35/43)、69.8%(30/43)和63.6%(21/33);安阳食管癌患者HPV16感染水平明显高于北京(H=3.91,P<0.05)和内蒙古(H=4.22,P<0.05)的食管癌患者。安阳食管癌患者中HPV16表达呈阳性、强阳性的比例明显高于北京和内蒙古食管癌患者(H=3.95,P<0.05)。结论HPV16在不同地区的食管癌患者中均有较高感染率。安阳食管癌高发区的患者感染HPV16的程度较严重。
BACKGROUND & OBJECTIVE: Anyang in Henan Province of China is a hyperendemic area of esophageal cancer. The infection of human papillomavirus (HPV) is thought as an important pathogenesis of esophageal cancer in Anyang. This study was to detect infection rate and level of HPV-16 in esophageal squamous cell carcinoma (ESCC) patients from 3 different Chinese areas, and investigate its relationship with the pathogenesis of ESCC. METHODS: Infection status of HPV-16 in 119 ESCC specimens (43 collected from Anyang, 43 from Beijing, and the rest 33 from Mongolia nationality of Inner Mongolia) was detected by in situ hybridization (ISH) technique with digoxin-labeled HPV-16 E6 probe. RESULTS: HPV16 infection rates were 81.4%, 69.8%, and 63.6% in the specimens from Anyang, Beijing, and Inner Mongolia, respectively. Infection level of HPV-16 was significantly higher in Anyang group than in Beijing group (H=3.91, P<0.05) and Inner Mongolia group(H=4.22,P<0.05). There was no significant difference between the latter 2 groups. Furthermore, the proportion of strong expression of HPV16 (++ and +++) was significantly higher in Anyang group than in the other 2 groups(H=3.95, P<0.05). CONCLUSIONS:HPV-16 infection rate is high in the esophageal specimens from the 3 different areas. Infection status of HPV16 is serious in Anyang.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2005年第7期870-873,共4页
Chinese Journal of Cancer
关键词
食管肿瘤
癌
鳞状细胞
乳头状瘤病毒/分离和提纯
原位杂交
检出率
人类
Esophageal neoplasms
Carcinoma, squamous cell
Papillomavirus/isolation and purification
In situ hybridization
Detection rate
Human
