摘要
目的:研究乳腺良、恶性病变组织中人乳头瘤病毒(HPVs)感染情况。方法:收集新鲜乳癌组织标本73例和乳腺良性病变组织标本84例,采用PCR方法对乳腺良、恶性病变组织中HPVs进行检测和分型。结果:73例乳癌组织中,HPV16阳性率为76.7%(56/73),高危型HPVs(HPV16和59)总阳性率76.7%(56/73);其中HPV16单独感染20例,HPV16、59混合感染6例,HPV16、6/11混和感染28例,3者混和感染2例。84例乳腺良性病变组织中,HPV16阳性率为48.8%(41/84),高危型HPVs总阳性率为51.2%(43/84);其中HPV16单独感染15例,HPV59单独感染2例,HPV6/11单独感染2例,HPV16、6/11混和感染24例,3者混和感染2例。乳腺良、恶性病变组织中均未检测到HPV18、31、33、52和58。乳癌组织中HPV16阳性率和高危型HPVs总阳性率高于乳腺良性病变(χ2分别为12.879,10.921,P<0.01)。浸润性导管癌组织中HPV16阳性率为86.0%(43/50),高于浸润性小叶癌组织中的56.6%(13/23)(χ2=7.663,P<0.01)。结论:HPV16在乳癌发生发展中可能具有重要作用,与乳癌组织学类型有关;其与其他HPVs亚型的混合感染值得重视。
Aim：To investigate the infection rate and typing of human papillomaviruses（HPVs） in breast benign and malignant lesions.Methods：A total of 73 fresh breast carcinoma tissue and 84 fresh breast benign lesions were collected and subjected to detect and type HPVs by PCR assay.Results：In 73 breast carcinoma tissue,the positive rate of HPV16 was 76.7%（56/73）,that of high risk-HPVs（HPV16 and 59） was 76.7%（56/73）,including 20 cases with HPV16 infection alone,6 cases with HPV16 and 59 mixed infection,28 cases with HPV16 and 6/11 mixed infection, and 2 cases with multiple infection （HPV16 with HPV59 and HPV6/11 ）. In 84 breast benign lesions, the positive rate of HPV16 was 48.8% （41/84） , that of high risk-HPVs was 51.2% （43/84） ,including 15 cases with HPV16 infection alone,2 cases with HPV59 infection alone ,2 cases with HPV6/11 infection alone ,24 cases with HPV16 and 6/11 mixed infection, 2 cases with multiple infections （HPVI6 with HPV59 and HPV6/11 ）. HPV18,31,33,52, and 58 were not found in all breast benign and malignant lesions. There was significant difference in the positive rates of HPV16 and HR-HPVs between breast benign and malignant lesions （χ^2 was 12. 879,10. 921 ,respectively,P 〈0.01 ）. The positive rate of HPV16 in invasive ductal carcinoma tissue was 80.6% （43/50） , higher than 56.6% （ 13/23 ） in invasive lobular carcinoma tissue （χ^2 = 7. 663, P 〈 0.01 ）. Conclusion ：HPV16 may play an important role in the carcinogenesis of breast carcinoma, and the mixed infection of HPV16 with HPV59 or HPV6/11 should be emphasized in breast carcinoma.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2009年第4期713-717,共5页
Journal of Zhengzhou University(Medical Sciences)
基金
卫生部科研基金资助项目WKJ2007-2-022
河南省高校杰出科研人才创新工程基金资助项目2007KYCX013
关键词
人乳头瘤病毒
乳癌
乳腺良性病变
human papillomavirus
breast carcinoma
breast benign lesion
