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HPV16 E7蛋白阳性和阴性乳癌组织中Rb和P16蛋白的表达 被引量：2

Expressions of Rb and P16 proteins in HPV16 E7 protein-positive and-negative breast carcinoma tissue
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摘要 目的:研究人乳头瘤病毒16型(HPV16)E7蛋白阳性(E7+)和阴性(E7-)乳癌组织中Rb和P16蛋白的表达及意义。方法:以经PCR筛选证实的HPV16 DNA阳性的56例乳癌组织(经Western blotting证实42例HPV16E7+,14例E7-)为研究对象,采用Western blotting方法检测癌组织中Rb和P16蛋白的表达。结果:HPV16E7+、HPV16E7-乳癌组织中,Rb蛋白阳性率分别为9.5%(4/42)、50.0%(7/14),P16蛋白阳性率分别为85.7%(36/42)、21.4%(3/14),HPV16E+和E7-乳癌组织中P16和Rb蛋白阳性率差异均有统计学意义(χ2=20.525,10.898,P均<0.001)。HPV16E7+乳癌组织中,Rb和P16蛋白表达有关(rp=0.792,P=0.003);HPV16E7-乳癌组织中,Rb和P16蛋白表达无关(P=0.182)。结论:HPV16E7可通过降解Rb,促进P16蛋白表达,从而在乳癌发生发展中发挥作用。 Aim：To investigate the expression and significance of Rb and P16 proteins in human papillomavirus 16 type（HPV16） E7 protein-positive（E7＋） and-negative（E7-） breast carcinoma tissues.Methods：A total of 56 HPV16 DNA-positive breast carcinoma specimens confirmed by PCR,including 42 HPV16 E7＋ and 14 HPV16 E7-breast carcinoma specimens certified by Western blotting assay,were subjected to detect Rb and P16 proteins by Western blotting assay.Results：The positive rates of Rb in HPV16 E7＋ and HPV16 E7-breast carcinoma tissues were 9. 5% （4/42） and 50.0% （7/14）, respectively, while the positive rates of P16 were 85.7% （36/42） and 21.4% （3/14）, respectively. There were significant differences in the expressions of P16 and Rb between HPVI6 E7^＋ and HPV16 E7 - tissue （χ2 was 20. 525,10. 898, respectively, P 〈 0.01 ）. The expression of P16 was also associated with that of Rb in HPV16 E7^＋ tissue （rp =0.792,P =0.003） ,but not in HPV16 E7- breast carcinoma tissue （P=0. 182）. Conclusion： The HPV16 E7 may play an important role in the carcinogenesis and progression of breast carcinoma by degrading Rb protein and up-regulating P16 protein expression.
作者 冶亚平 千新来 贺国洋 常海敏 朱慧芳 韩芳毅 杨慧林 王霞 赵杰 李新强 张洁
机构地区 新乡医学院病理学教研室 新乡医学院法医临床学教研室
出处 《郑州大学学报（医学版）》 CAS 北大核心 2009年第4期723-725,共3页 Journal of Zhengzhou University(Medical Sciences)
基金 卫生部科研基金资助项目WKJ2007-2-022 河南省高校杰出科研人才创新工程资助基金项目2007KYCX013
关键词 人乳头瘤病毒16型 早期基因7 RB蛋白 P16蛋白 乳癌 human papillomavirus 16 type early gene 7 Rb p16 breast carcinoma
分类号 R737.9 [医药卫生—肿瘤]
  • 相关文献

参考文献11

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二级参考文献8

  • 1Brown C, Knwalczyk AM, Taylor ER, et al. P53 represses human papillomavirus type 16 DNA replication via the viral E2 protein[ J]. Virol J,2008,5:5.
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  • 8牛永东,李恩民,吴贤英,郑瑞明,沈忠英,许丽艳.食管鳞癌中HPV-16/18感染与c-erbB-2、p53和Rb表达及其意义[J].肿瘤防治杂志,2002,9(2):138-141. 被引量：7

共引文献3

同被引文献18

  • 1Narisawa-Saito M, Kiyono T. Basic mechanisms of high-risk human papillomavirus induced carcinogenesis: Roles of E6 and E7 proteins[J]. Cancer Sci,2007,98(10) :1 505.
  • 2Chakrabarti O, Krishna S. Molecular interactions of "high risk" human papillomaviruses E6 and E7 oncoproteins: implications for tumour progression [ J ]. J Biosci, 2003,28 (3) :337.
  • 3Zur Hausen H. Papillomaviruses and cancer from basic studies to clinical application [ J ]. Nat Rev Cancer,2002,2 (5) :342.
  • 4Liu X, Clements A, Zhao K, et al. Structure of the human Papillomavirus E7 oncoprotein and its mechanism for inactivation of the retinoblastoma tumor suppressor[ J]. J Biol Chem,2006, 281(1) : 578.
  • 5Ivanova TA, Golovina DA, Zavalishina LE, et al. Up-regulation of expression and lack of 5' CpG island hypermethylation of p16 INK4a in HPV-positive cervical carcinomas [J]. BMC Cancer,2007,7:47.
  • 6Sharma G, Mirza S, Prasad CP, et al. Promoter hypermethylation of p16INK4A, p14ARF, CyclinD2 and Slit2 in serum and tumor DNA from breast cancer patients [ J]. Life Sci,2007,80(20) :1 873.
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  • 8Liu T, Niu Y, Feng Y, et al. Methylation of CpG islands of p16 ( INK4a ) and cyclinD1 overexpression associated with progression of intraductal proliferative lesions of the breast [J]. Hum Pathol,2008,Jul 24. [Epub ahead of print].
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  • 10Kalof A, Cooper K. p16INK4a immunoexpression : surrogate marker of high-risk HPV and high-grade cervical intraepithelial neoplasia[J]. Adv Anat Pathol, 2006,13(4) :190.

引证文献2

二级引证文献19

郑州大学学报（医学版）
2009年 第4期

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