摘要
诸葛菜的花瓣、萼片、花丝在ＭＳ附加６—ＢＡ与２，４—Ｄ的培养基上培养７～１０ｄ，诱导形成愈伤组织。愈伤组织在ＭＳ＋ＮＡＡ１ｍｇ／Ｌ＋６－ＢＡ３ｍｇ／Ｌ的分化培养基上培养７ｄ左右分化出芽，每块愈伤组织形成３～４个芽。花托、花序轴切段在分化培养基上可直接出芽形成植株。
The floral parts of Orychophragmus violaceus showed apparent regeneration capacity through culture in vitro. The calli differentiation of petal, sepal and filament were realized which they were cultivated in MS medium supplemented with 2.4-D (0.5mg/L)and 6-BA (0.5mg/L). Increasing NAA(1mg/L) and 6-BA (0.5mg/L) concentration in MS medium induced plant regeneration. The plants could be obtained from cultivation of receptacle and rachis in differentiation medium.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
1998年第2期7-9,共3页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金
关键词
诸葛菜
花器培养
再生植株
组织培养
Orychophragmus violaceus Tissue culture Floral parts
