摘要
湖北诸葛菜花托、花丝在 ＭＳ＋０．５～５ｍｇ／Ｌ６－ＢＡ＋０．２ｍｇ／ＬＮＡＡ的培养基上直接出 芽，形成愈植株．无菌苗叶柄、叶片在附加 ６－ＢＡ或ＫＴ与ＮＡＡ不同浓度及组合的 ＭＳ培养基上 诱导形成伤组织或胚状体，愈伤组织分化出芽．再生植株在ＭＳ＋３ｍｇ／Ｌ ６－ＢＡ＋０．３ｍｇ／Ｌ ＮＡＡ培 养基上现蕾开花．
Regeneration small buds wer induced from receptacles ac filaments of Orychophragmus hupehensis cultured on MS medium with 3mg/L 6-BA + 0. 2mg/L NAA, which later developed into plantlets.Its petioles and leaves were better explants for inducing calli or somatic embryogenesis on MS medium with different concentration of 6-BA or KT and NAA. The calli and somatic embryogeesis later developed into shoot clusters. The plantlet from receptacles blooms on MS medium with 3mg/L 6- BA+0. 2mg/L NAA.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第1期103-106,共4页
Journal of Sichuan University(Natural Science Edition)
基金
国家自然科学基金!39870080
