摘要
以桂竹香花柄、花萼、花瓣、花丝为外植体 ,研究不同激素对诱导愈伤组织和不定芽的影响。研究结果表明 ,4种外植体均能形成愈伤组织 ,但愈伤组织的发生率、发生部位及形态等表现差异。花柄愈伤组织继代MS + 2 ,4 D +BA培养基 ,可分化产生不定芽 ,出芽率 78%～ 82 %。再生小苗在 1/ 2MS +NAA 0 .
The calli were induced from flower organs such as pedicel, calyx, petal and filament of Cheiranthus cheiril L. on MS basal medium supplemented with auxins. The effects of auxins and explants on callus induction and adventitious bud differentiated were investigated. The callus fromation percentage, morphogenesis, position and days were different in four explans and auxins treatment. The callus from pedicel explant produced adventitious bud by subculture on MS+2,4 D+BA, which rate were 78%～82%. The regeneration plant were formed after rooting on MS+NAA 0.5mg/L.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2001年第1期34-37,共4页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金资助项目! (89870 0 80 )
