摘要
目的:对泻心汤(大黄、黄芩和黄连)煎煮过程中产生的沉淀物采用LC/MS2法进行成分分析。方法:采用液相色谱-质谱法,C18柱,以乙腈-醋酸盐缓冲液进行梯度洗脱。DAD在线检测波长为270nm。结果:泻心汤沉淀物与其上清液所含化合物组成基本接近,泻心汤沉淀物在人工胃液、人工肠液中的溶解性呈现出一定的规律性。结论:泻心汤沉淀物与其上清液在疾病治疗上同等重要,在临床使用该方剂时需注意到这个事实。
AIM： To study the ingredients of sediments from Xiexin Decoction（XXD） （Radix et Rhizoma Rhei; Radix scutellariae and Rhizoma coptidis） with the method of LC/MS2. METHODS： A C18 column was used, with acetonitrile-acetate buffer as mobile phase, and the online detection wavelength was 270 rim. RESULTS： The ingredients of sediments from XXD were nearly the same as its supernatant fluid, There were the similarities between the solubilities of sediments from XXD in artificial gastric juice and in artificial intestinal fluid. CONCLUSION： In clinical trials we should pay much attention to this fact that sediments from XXD and its supernatant fluid have similarly importance.
出处
《中成药》
CAS
CSCD
北大核心
2009年第8期1250-1254,共5页
Chinese Traditional Patent Medicine
关键词
泻心汤
沉淀物
成分分析
Xiexin Decoction
sediments
ingredient analysis
