摘要
采用HPLC-MS分别对中药附子配伍甘草前后汤液中产生的沉积物进行化学组成鉴定,探讨附子配伍甘草减毒的可能机制,并为含附子-甘草药对的中药成方制剂的提取纯化工艺合理性提供实验依据。分别制备附子单煎液沉积物与附子甘草合煎液沉积物供试品,在同一分析条件下,分别建立了其HPLC-MS指纹图谱,并采用Q-TOFMS检测。从附子单煎液沉积物中指认出28种成分(鉴定出25种成分及结构);从附子甘草合煎液沉积物中指认出36种成分(鉴定出34种成分及结构),其中11种来源于甘草,25种成分来源附子,但附子配伍甘草前后汤液沉积物中生物碱成分种类和含量差别均较大。说明配伍甘草可能导致汤液中附子生物碱成分状态的改变,为附子-甘草配伍减毒机制的探讨提供了实验依据,同时也提示附子-甘草药对煎煮液沉积物中含有效成分,在临床和实际生产中需注意沉积物部分的综合利用。
The chemical ingredients of sediments from single Radix Aconiti Lateralis （Fuzi, in Chinese） decoction and Radix Aconiti Lateralis-Radix Glycyrrhizae （Gancao, in Chinese） decoction were evaluated separately by HPLC-MS for exploring their ingredients difference and discussing the mechanism of toxicity reduction of Fuzi-Gaocao herb-pair, even providing the experimental basis for extracting and purifying process of TCM preparations containing Fuzi-Gancao herb-pair. The sediments samples from single Fuzi decoction （FD） and Fuzi-Gaocao decoction （FGD） were prepared separately firstly. Then these samples were analyzed in the same chromatographic and mass spectrometry condition, estimating their HPLC-MS fingerprint and identifying their compounds by Q-TOFMS. As a result, 28 compounds in sediments samples from FD were obtained, among which 25 compounds and structures were confirmed. Besides, 36 compounds in sediments samples from FGD sample were obtained including 34 confirmed compounds and structures, among which there were 11 compounds from Gancao and 25 compounds from Fuzi. However, according to these confirmed compounds, alkaloids compounds in sediments samples from FGD were significantly different from that in sediments samples from FD. This result showed the fact that there would be some influences on alkaloid compounds in decoction after the combination of Gancao. In this study, the experiment basis of the mechanism of toxicity reduction of Fuzi-Gaocao herb-pair is provided. It also showed that much attention should be paid to the fact that there were great amount of alkaloid compounds, the effective compounds, in the sediments for which a comprehensive utilization in clinic and pharmaceutical process should be noted.
出处
《药学学报》
CAS
CSCD
北大核心
2012年第11期1527-1533,共7页
Acta Pharmaceutica Sinica
基金
国家自然科学基金面上项目(81073070)
关键词
附子
甘草
沉积物
HPLC-MS
化学组成
Radix Aconiti Lateralis
Radix Glycyrrhizae
sediment
HPLC-MS
chemical ingredient
