摘要
目的研究壳聚糖镁膜与PC12细胞体外生物相容性,初步探讨壳聚糖/镁复合物作为组织工程材料的可行性。方法合成壳聚糖-镁膜,应用扫描电镜观察复合膜的表面形态,并用X线能谱仪分析壳聚糖-镁复合膜中镁的含量;在体外将复合膜与PC12细胞共培养,用MTT方法检测细胞活力。光镜及扫描电镜观察PC12细胞在材料上的形态学变化。结果单纯壳聚糖(CS)与壳聚糖镁膜(CM)组相比,前者表面较为光滑致密,而复合膜中可见均匀排列的小孔;络合物中镁元素的含量与投入的硫酸镁量呈一定的剂量依赖。形态学观察表明,PC12细胞在CM上生长良好,至7d时,可见微绒毛丰富并有较长突起,部分细胞之间形成突触状结构;MTT检测结果表明,培养5d后实验组细胞活力超过对照组(P<0.05)。结论壳聚糖/镁膜与PC12细胞在体外有良好的生物相容性。
Objective To prepare the chitosan-Mg membranes and to explore the biocompatibility of membranes with PCI2 cells and its usage as biomaterials in tissue engineering. Methods The appearance of the chitosan-Mg membranes （CM） was observed under scanning electromicroscope （SEM） and the element of the membranes was analyzed by x-ray energy spectromete. PC12 ceils were co-cultured with CM in vitro, the morphological changes of PC12 cells on membranes were observed under scanning electron microscope （SEM） and light microscope （LM）, the cell vitality was detected by MTT assay. Results The surface of chitosan membranes （CS） was smoother than that of CM ;but the CM groups were full of pore observed under SEM;The content of Mg element was related with the dosage of MgSO4 added into chitosan solution. Morphology observation showed that PCl2 cells grew well on CM as compared with CS group. The cells were rich of microvilli and long processes on 7th day, and synapses-like structure was formed in many PC12 cells. In addition, the cell viability of experiment group was higher than that of control group（P 〈 0. 05）after 5 days. Conclusion Chitosan-Mg membrances has good biocompatibility with PC12 cells.
出处
《基础医学与临床》
CSCD
北大核心
2009年第8期840-844,共5页
Basic and Clinical Medicine
基金
南通大学自然科学基金(06Z117)
