摘要
目的评价快速成型工艺制作的羟基磷灰石/聚乳酸-聚羟乙酸/骨形态发生蛋白(HA/PLGA/BMP-2)生物材料在新西兰兔体内的生物组织相容性。方法健康新西兰大白兔20只,随机抽取10只为实验组,10只为对照组。实验组于兔颅顶部植入HA/PLGA/BMP-2生物材料,对照组植入金属钛网。分别于4周、8周、12周通过HE染色、血常规、血生化等评价方法,评价HA/PLGA/BMP-2生物材料在兔体内的组织相容性。结果实验组植入物周围组织(约1 cm)HE染色结果显示:4周见部分中性粒细胞,少量炎性细胞浸润;8周未见中性粒细胞,无炎性细胞浸润;12周未见炎性细胞,周围可见成纤维细胞增生。对照组植入物周围组织(约1 cm)HE染色结果显示4周、8周、12周均未见有炎性细胞生成。实验组、对照组肝、肾组织HE染色结果显示均未见有炎性细胞,无新生组织形成。实验组与对照组12周血液学结果比较,差异无显著性意义。结论 HA/PLGA/BMP-2生物材料具有良好的生物相容性,是一种安全、无肝肾毒性的生物材料,在新型骨组织工程材料上有一定的研发潜力。
Objective To evaluate the histocompatibility of the biomaterial made using rapid prototyping technology, HA/PLGA/BMP-2 complexes, in New Zealand rabbits in vivo. Methods Twenty New Zealand rabbits were randomly divided into 2 groups, 10 rabbits in experimental group and the other 10 rabbits in control group. Rabbits in the experimental group were implanted with HA/PLGA/BMP-2 complexes into muscles of the skull. Meanwhile, titanium cages were implanted into the same position of rabbits in control group. The histoeompatibility of HA/PLGA/BMP-2 complexes in rabbits in vivo was evaluated using HE staining and tests of blood routine and biochemistry at the 4t~, 8th, and 12^th week, respectively. Results HE staining results of the tissue around implantation （approx. l cm） in experimental group indicated that some neutrophilic granuloeytes and few inflammatory cells infiltrated at the 4^th week. No neutrophilic granulocytes and inflammatory cells were observed at the 8th week. And no neutrophilic granulocyte but only fibroblast hyperplasia was observed at the 12^th week. No inflammatory cell was observed in HE staining in control group at the 4^th, 8^th, and 12^th week, respectively. The HE staining results of the liver and kidney in both groups showed no inflammatory cells or new tissue formation. The results of blood test between two groups at 12^th week showed no significant difference. Conclusion HA/PLGA/BMP-2 has good histocompatibility, with safe characteristics and no liver and renal toxicity. It has great potential in theosseous tissue engineering research.
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2013年第4期336-339,374,共5页
Chinese Journal of Osteoporosis
基金
全军十二五课题(11MS016)
