摘要
目的 研究镁离子(Mg2+)对神经元保护的机制。方法 采用全细胞膜片钳技术,研究Mg2+对培养的大鼠海马神经元GABA_A受体介导电流的作用。结果 (1)在钳制电压为-40mV时,GABA可通过GABA_A受体介导产生内向电流;(2)当GABA和Mg2+同时作用时,该电流峰值增高;(3)在Mg2+存在的情况下,GABA的浓度-效应曲线平行左移。结论 Mg2+通过正性变构调节机制影响GABA_A受体的功能,达到保护神经元的作用。
Objective To investigate the protective mechanism of Mg2f on neuron. Methods GABAA-re-ceptor mediating responses in cultured rat hippocampal neurons were studied using whole-cell recording techniques. Results (1) GABA induced inward currents through activation of GABAA-receptor at a holding potential of 40mV; (2) GABA_A-receptor mediated responses were enhanced when Mg2+ and GABA used together ; (3) in the presence of Mg2+ , the concentration-response curve of GABA-induced responses was shifted to the left in parallel manner. Conclusion It is implicated that Mg2+ affect the function of GABAA-receptor through positive al-losteric manner. By this manner,it protects the ischemic neurons.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2002年第6期326-328,共3页
Journal of Apoplexy and Nervous Diseases
基金
国家自然科学基金资助项目(No
30040037)
