摘要
目的:总结周围神经组织工程生物支架材料生物相容性评价方法的进展。资料来源:应用计算机检索CNKI1990-01/2006-12有关周围神经组织工程生物材料生物相容性评价方面的文章,检索词“神经,组织工程,生物材料,生物相容性”,限定文献语言种类为中文。同时计算机检索ElsevierScienceE-journals1990-01/2006-12有关周围神经组织工程生物材料生物相容性评价方面的文章,检索词“nerve,tissueengineering,biomaterial,biocompatibility”,限定文献语言种类为English。资料选择:对资料进行初审,选取周围神经组织工程生物材料方面的相关文献,开始查找全文。排除中枢神经系统文献。对剩余的文献进行分析,总结研究内容。资料提炼:共收集到相关文献168篇,72篇符合纳入标准,其中30篇有关周围神经组织工程生物材料生物相容性评价方面的文献用于综述。资料综合:周围神经组织工程生物材料生物相容性评价可分为体外和体内两个方面。体外评价即利用生物材料与细胞共培养,通过观察细胞的形态,测定细胞的活力和凋亡等反映细胞的细胞毒性。体内评价最常用大鼠坐骨神经模型,通过普通和免疫组化染色、血液指标检测、器官病理切片等反映生物材料体内局部和整体的相容性。目前,生物相容性评价以短期为主,而其长期评价也是必须的。结论:在常用方法的基础上,一些新的方法逐渐被运用到周围神经组织工程支架材料的生物相容性评价上。对于各种评价方法的“性价比”还需要进一步的分析,以建立一套基本评价体系。
OBJECTIVE： To review the progress of the methods for the biocompatibility estimation of peripheral nerve tissue engineering scaffold biomaterials.
DATA SOOURCES： A computer-based search was conducted in CNKI and ElsevierScience E-journals for articles related to biocompatibility estimation of peripheral nerve tissue engineering biomaterials from January 1990 to December 2006 with the key words of ＂nerve, tissue engineering, biomaterials, and biocompatibility＂.
STUDY SELECTION： Data were check firstly. Articles related to peripheral nerve tissue engineering biomaterials were selected and looked for full-texts. Researches on central nervous system were excluded. The rest articles were analyzed and summarized.
DATA EXTRACTION： One hundred and sixty-eight related articles were selected in all, among which 72 met the inclusive criteria. Thirty articles about biocompatibility estimation of peripheral nerve tissue engineering biomaterials were collected for review.
DATA SYNTHESIS： Biocompatibility estimation of peripheral nerve tissue engineering biomaterials can be divided into two aspects： in vitro and in vivo. In vitro, the coculture of biomaterials and cells is used to reflect biomaterials＇ cell toxicity by detecting cells＇ morphology, viability, apoptosis and so on. In vivo, rat＇s sciatic nerve models are most commonly used. Biocompatibility in local and systematic level can be monitored by certain and immunohistochemical staining, hematological indexes, organs＇ pathological section and so on. Recently, biocompatibility estimation is mostly a shot time process; however, a long period estimation must be done.
CONCLUSION： Based on the common methods, some novel approaches are used for biocompatibility estimation of peripheral nerve tissue engineering biomaterials gradually. To establish a basic estimation system, the ratio of ＂efficiency and cost＂ needs further analyze for various estimation methods.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第9期1719-1723,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
