摘要
克隆于细菌表达载体ｐＧＥＭ－３ｚｆ（－）中的ＡＦＰ基因经ＳａｌＩ和ＫｐｎＩ消化后，插入到热激盒式表达载体ｐＭＡ４１２的ＳａｌＩ和ＫｐｎＩ位点中，连接于一可融合的报道基因（β－葡萄糖醛酸苷酶基因）之后，共同受玉米热激蛋白启动子控制，构成一表达单元．并将此带有ＡＦＰ基因的热激盒式表达单元亚克隆于植物双元表达载体ｐＢＩｎ１９中，成功地构建成ｐＸＭ０５，进而构建了ｐＸＭ１５表达载体．
Antifreeze peptide gene cloned in the pXQ04 was digested by SalI/KpnI,inserted into the SalI/KpnI site of heat shock expression vector pXM412 and fused with a reporter gene-gus and was under the control of heat shock promoter.Then we subcloned BglⅡ fragment of pHSP-Gus-AFP from pXM05 into binary vector pBIN19.These subcloned were confirmed by restriction enzyme digestion analysis and PCR.
出处
《内蒙古大学学报（自然科学版）》
CAS
CSCD
1998年第4期541-543,共3页
Journal of Inner Mongolia University：Natural Science Edition
基金
国家自然科学基金
关键词
抗冻肽基因
热激启动子
构建
基因表达
antifreeze peptide gene,heat shock promoter,construction
