摘要
将整合在Ｔｉ质粒上的ａｆｐ基因作为供体ＤＮＡ，用花粉管通道和子房注射方法导入番茄品种“中蔬四号”中，对Ｄ１、Ｄ２代进行Ｓｏｕｔｈｅｒｎ杂交获得杂交带；田间抗寒性试验表明，在春季平均气温低于正常年份４·４℃条件下，转基因组植株生长势优于对照；致死温度也比对照降低２℃．
Antifreeze protein gene(afp)which is integrated into the Ti plasmid pMon322,was introduced into tomato via the pollen tube pathway and ovary injection.The transgenic plants of D 1 and D 2 generation produced afp DNA on Southern blot.The assay of cold resistance in the filed showed that transgenic plants could grow better than control under the average temperature that is 4.4℃ below normal year in earlier spring,meanwhile the fatal temperature dropped 2℃.These results revealed that AFP gene has been integrated into the transgenic plants genome and expressed.
基金
国家自然科学基金资助项目(39O7O595)
